Concanavalin A from Canavalia ensiformis (Jack bean)

peroxidase conjugate, lyophilized powder


Quality Level

biological source

Canavalia ensiformis (jack bean)


lyophilized powder


blood typing: suitable
fractionation: suitable


carbohydrate, essentially free


peroxidase conjugate

storage temp.


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General Western Blot Protocol:
  • Glycoprotein sample size: 500ng
  • Lectin Concentration: 0.1ug/ml

  1. Load samples at 500 ng of glycoprotein per lane
  2. Run 4-20% Bis-Tris SDS page gel
  3. Transfer gel to a PVDF membrane
  4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
  5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
  6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
  7. Detect using chemiluminescent substrate (CPS1-120)
Detects glycoproteins containing α-D-mannose, α-D-glucose when used with appropriate peroxidase substrate

Biochem/physiol Actions

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.


Package size based on protein content

Unit Definition

One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 °C.

Physical form

Lyophilized powder containing tris-citrate buffer salts and trace calcium and manganese

Preparation Note

Prepared from peroxidase type VI (P8375), using the method of Wilson and Nakane, which promotes conjugation but prevents the interaction between Con A and peroxidase sugar residues.

Analysis Note

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.


Health hazard




Repr. 2 - Resp. Sens. 1 - Skin Sens. 1


11 - Combustible Solids

WGK Germany


Flash Point F

Not applicable

Flash Point C

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Wilson, M.B. and Nakane, P.K. et al.
Immunofluorescence and Related Staining Techniques, 215-215 (1978)
Johanna Kabbert et al.
The Journal of experimental medicine, 217(11) (2020-07-09)
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