Merck
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L6529

Sigma-Aldrich

Lipopolysaccharides from Escherichia coli O55:B5

γ-irradiated, BioXtra, suitable for cell culture

Synonym(s):
LPS
EC Number:
MDL number:

Quality Level

sterility

γ-irradiated

product line

BioXtra

form

lyophilized powder

purified by

gel-filtration chromatography

technique(s)

cell culture | mammalian: suitable

impurities

<3% Protein (Lowry)

solubility

H2O: 5 mg/mL, faintly hazy to hazy

storage temp.

2-8°C

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Related Categories

General description

Lipopolysaccharides (LPS) are complex glycolipids that contain several components. Enterobacteriaceae, such as E. coli, have highly conserved lipid A linked to the antigenic O-polysaccharide. Hydrophobic lipid A forms the outer most component that plays a role in the biological properties attributed to endotoxic LPS. A short core oligosaccharide (core OS) is found to be attached to lipid A. Enterobacteria contain an antigenic, repeat-structure O-polysaccharide (O-PS) attached to the distal core OS to form smooth LPS. O-PS in E. coli contains ∼170 distinct antigens that interact with the host immune system. Based on the core OS structures in E. coli, they have been designated K-12 and R1 to R4.

Application

LPS (lipopolysaccharide) from Escherichia coli 055:B5 has been used:
  • to stimulate human PBMC to secrete cytokine.
  • to stimulate wild type embryonic fibroblasts and mutants for the induction of phosphorylation of p56
  • in the measurement of tumor necrosis factor-α levels and nuclear factor-κB p65 activities after stimulation
  • in the stimulation of hamster lymphocytes in vitro
  • to induce activated dendritic morphology in murine macrophage cell line in a study
Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Biochem/physiol Actions

LPS (lipopolysaccharide) is a major constituent of the outer membrane of most gram negative bacteria. It is a highly immunogenic antigen with the ability to enhance immune responses to soluble antigens. The LPS confers antigenic and endotoxic characters to the bacteria. They are also important in resistance to infection by phages.

Reconstitution

Lipopolysaccharides are supplied as lyophilized, γ-irradiated powders. To reconstitute, add 1 ml sterile balanced salt solution or tissue culture medium to the vial (1 mg) and gently swirl until the powder dissolves. Reconstituted product may be further diluted to desired working concentrations using sterile balanced salt solution or tissue culture medium.

Pictograms

Skull and crossbones

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 2 Oral

Storage Class Code

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Quotes and Ordering

Natalie M Otto et al.
Critical care (London, England), 12(4), R107-R107 (2008-08-20)
Hyperglycemia is associated with negative outcomes in various settings of critical illness; infectious complications, especially, seem to be increased. On the other hand, intensive insulin therapy (IIT) has been shown to improve outcome in clinical trials. Whether normoglycemia itself or
Cheng Yang Ng et al.
Scientific reports, 7(1), 13683-13683 (2017-10-24)
Cytosolic phospholipase A
PURIFICATION AND CHARACTERIZATION OF THE LIPID A COMPONENT OF THE LIPOPOLYSACCHARIDES FROM ESCHERICHIA COLI.
A J BURTON et al.
Biochemistry, 3, 411-418 (1964-03-01)
D E Heinrichs et al.
Molecular microbiology, 30(2), 221-232 (1998-10-29)
Bacterial lipopolysaccharides (LPS) are unique and complex glycolipids that provide characteristic components of the outer membranes of Gram-negative bacteria. In LPS of the Enterobacteriaceae, the core oligosaccharide links a highly conserved lipid A to the antigenic O-polysaccharide. Structural diversity in
Katerina Akassoglou et al.
Proceedings of the National Academy of Sciences of the United States of America, 101(17), 6698-6703 (2004-04-21)
In multiple sclerosis, in which brain tissue becomes permeable to blood proteins, extravascular fibrin deposition correlates with sites of inflammatory demyelination and axonal damage. To examine the role of fibrin in neuroinflammatory demyelination, we depleted fibrin in two tumor necrosis

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