All Photos(1)



Lipopolysaccharides from Escherichia coli O55:B5

γ-irradiated, BioXtra, suitable for cell culture

EC Number:
MDL number:

Quality Level



product line



lyophilized powder

purified by

gel-filtration chromatography


cell culture | mammalian: suitable


<3% Protein (Lowry)


H2O: 5 mg/mL, faintly hazy to hazy

storage temp.


Looking for similar products? Visit Product Comparison Guide

General description

Lipopolysaccharides (LPS) are complex glycolipids that contain several components. Enterobacteriaceae, such as E. coli, have highly conserved lipid A linked to the antigenic O-polysaccharide. Hydrophobic lipid A forms the outer most component that plays a role in the biological properties attributed to endotoxic LPS. A short core oligosaccharide (core OS) is found to be attached to lipid A. Enterobacteria contain an antigenic, repeat-structure O-polysaccharide (O-PS) attached to the distal core OS to form smooth LPS. O-PS in E. coli contains ∼170 distinct antigens that interact with the host immune system. Based on the core OS structures in E. coli, they have been designated K-12 and R1 to R4.


LPS (lipopolysaccharide) from Escherichia coli 055:B5 has been used:
  • to stimulate human PBMC to secrete cytokine.
  • to stimulate wild type embryonic fibroblasts and mutants for the induction of phosphorylation of p56
  • in the measurement of tumor necrosis factor-α levels and nuclear factor-κB p65 activities after stimulation
  • in the stimulation of hamster lymphocytes in vitro
  • to induce activated dendritic morphology in murine macrophage cell line in a study
Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Biochem/physiol Actions

LPS (lipopolysaccharide) is a major constituent of the outer membrane of most gram negative bacteria. It is a highly immunogenic antigen with the ability to enhance immune responses to soluble antigens. The LPS confers antigenic and endotoxic characters to the bacteria. They are also important in resistance to infection by phages.


Lipopolysaccharides are supplied as lyophilized, γ-irradiated powders. To reconstitute, add 1 ml sterile balanced salt solution or tissue culture medium to the vial (1 mg) and gently swirl until the powder dissolves. Reconstituted product may be further diluted to desired working concentrations using sterile balanced salt solution or tissue culture medium.


Skull and crossbones

Signal Word


Hazard Statements

Hazard Classifications

Acute Tox. 2 Oral

Storage Class Code

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

A J BURTON et al.
Biochemistry, 3, 411-418 (1964-03-01)
Christopher D Hammerbeck et al.
Journal of virology, 85(19), 9929-9944 (2011-07-22)
Andes virus (ANDV) is associated with a lethal vascular leak syndrome in humans termed hantavirus pulmonary syndrome (HPS). In hamsters, ANDV causes a respiratory distress syndrome closely resembling human HPS. The mechanism for the massive vascular leakage associated with HPS
K Amor et al.
Infection and immunity, 68(3), 1116-1124 (2000-02-26)
In the lipopolysaccharides of Escherichia coli there are five distinct core oligosaccharide (core OS) structures, designated K-12 and R1 to R4. The objective of this work was to determine the prevalences of these core OS types within the species. Unique
Mazhar Adli et al.
The Journal of biological chemistry, 281(37), 26976-26984 (2006-07-15)
Nuclear factor kappaB (NF-kappaB) has been studied extensively as an inducible transcriptional regulator of the immune and inflammatory response. NF-kappaB activation downstream of lipopolysaccharide or cytokine stimulation is controlled by the IkappaB kinase complex, which contains IKKalpha and IKKbeta. Significantly
Cheng Yang Ng et al.
Scientific reports, 7(1), 13683-13683 (2017-10-24)
Cytosolic phospholipase A

Related Content

Human Microbiome Research Workflow: An Overview of the Role in Human Health and Disease

An overview of human microbiome research, workflow challenges, sequencing, library production, data analysis, and available microbiome reagents to support your research.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service