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M1028

Sigma-Aldrich

Magnesium chloride solution

for molecular biology, 1.00 M±0.01 M

Linear Formula:
MgCl2
CAS Number:
Molecular Weight:
95.21
MDL number:
PubChem Substance ID:
NACRES:
NA.52

Quality Level

grade

for molecular biology

sterility

non-sterile; 0.2 μm filtered

form

liquid

concentration

1.00 M±0.01 M

cation traces

heavy metals (as Pb): ≤5 ppm

foreign activity

DNase, RNase, NICKase and protease, none detected

SMILES string

Cl[Mg]Cl

InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2

InChI key

TWRXJAOTZQYOKJ-UHFFFAOYSA-L

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General description

Magnesium is a typical ionic halide, and is very soluble in water. Magnesium has a variety of biological roles in enzymology, cell membrane/wall structure, muscle cell physiology, and nucleic acid structure. Magnesium is an essential co-factor in many enzymes, including DNAse, some restriction enzymes, and Ribonuclease H.

Magnesium chloride is widely used to supply the magnesium ion in various molecular biology applications, including PCR reactions.

Application

Magnesium chloride is widely used to supply the magnesium ion in various molecular biology applications, including PCR reactions and buffers. It was used to prepare buffer to lyse cells for immunoprecipitation and to prepare serum-free medium for electrophysiological studies of rat brain slice cultures.

Components

Magnesium chloride solution contains 1M magnesium chloride in 18 megaohm water.

Storage Class Code

12 - Non Combustible Liquids

WGK Germany

WGK 1

Flash Point F

Not applicable

Flash Point C

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Yelena Y Grinberg et al.
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Antimo Migliaccio et al.
Methods in molecular biology (Clifton, N.J.), 776, 361-370 (2011-07-29)
Much evidence indicates that, with few exceptions, non-genomic actions of steroids are mediated by receptors universally known as nuclear receptors. Steroid receptors do not exhibit intrinsic tyrosine kinase activity. Nevertheless, they stimulate different signaling pathways in cytoplasm of target cells...
Bert De Rybel et al.
Science (New York, N.Y.), 345(6197), 1255215-1255215 (2014-08-12)
Coordination of cell division and pattern formation is central to tissue and organ development, particularly in plants where walls prevent cell migration. Auxin and cytokinin are both critical for division and patterning, but it is unknown how these hormones converge...
Hideyuki Arita et al.
Brain tumor pathology, 32(1), 22-30 (2014-04-22)
Assessment of the mutational status of the isocitrate dehydrogenase 1/2 (IDH1/2) gene has become an integral part of the standard diagnostic procedure and, therefore, needs to be accurate. This may, however, be compromised by various factors including the method of...
Susanne Hellmuth et al.
The EMBO journal, 33(10), 1134-1147 (2014-05-02)
The universal triggering event of eukaryotic chromosome segregation is cleavage of centromeric cohesin by separase. Prior to anaphase, most separase is kept inactive by association with securin. Protein phosphatase 2A (PP2A) constitutes another binding partner of human separase, but the...

Protocols

Enzymatic Assay of Mutanolysin

This procedure may be used for Mutanolysin products.

Enzymatic Assay of Hexokinase

Objective: To standardize a procedure for the enzymatic determination of Hexokinase.

Enzymatic Assay of Lipase Type XIII from Pseudomonas sp. Using a Coupled Enzyme System of Glycerol Kinase and Glycerophosphate Oxidase (EC 3.1.1.3)

Enzymatic assay of lipase type XIII from Pseudomonas sp. using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC 3.1.1.3)

Related Content

Multiplex qPCR with JumpStart™ Taq ReadyMix™ for Quantitative PCR

Method outlines use of a hot start Taq for multiplex qPCR and provides guidance on how to optimize dNTPs, primer, probes and MgCL2 concentrations. By optimizing these parameters, the user can improve assay sensitivity and linear range of detection.

RNAi In Situ

In Situ Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry. This is a protocol describing how to perform in situ hybridization on whole mouse embryos. Here we describe the hybridization procedure, and the localization of the DIG-labeled RNA using a conjugate of anti-DIG Fab antibody and calf intestinal alkaline phosphatase. Enzyme activity of the reporter is detected by a color reaction, resulting in the formation of a water-insoluble purple/blue precipitate. Manipulating the Mouse Embryo - Third Edition

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