for molecular biology
non-sterile; 0.2 μm filtered
1.00 M±0.01 M
heavy metals (as Pb): ≤5 ppm
DNase, RNase, NICKase and protease, none detected
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This procedure may be used for Mutanolysin products.
Objective: To standardize a procedure for the enzymatic determination of Hexokinase.
Enzymatic assay of lipase type XIII from Pseudomonas sp. using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC 22.214.171.124)
Method outlines use of a hot start Taq for multiplex qPCR and provides guidance on how to optimize dNTPs, primer, probes and MgCL2 concentrations. By optimizing these parameters, the user can improve assay sensitivity and linear range of detection.
In Situ Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry. This is a protocol describing how to perform in situ hybridization on whole mouse embryos. Here we describe the hybridization procedure, and the localization of the DIG-labeled RNA using a conjugate of anti-DIG Fab antibody and calf intestinal alkaline phosphatase. Enzyme activity of the reporter is detected by a color reaction, resulting in the formation of a water-insoluble purple/blue precipitate. Manipulating the Mouse Embryo - Third Edition