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McCoy′s 5A Medium

Modified, with L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

5A basal medium
MDL number:

Quality Level




cell culture | mammalian: suitable


NaHCO3: no
L-glutamine: 0.21915 g/L
glucose: 3.0 g/L (Dextro)
phenol red: 0.011 g/L

shipped in


storage temp.


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Used for the primary culture of cells from a wide range of tissues. Recommend use for production of virus in primary cell cultures.

Biochem/physiol Actions

Originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also be used in the culture of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.


Formulated to contain 11.9 grams of powder per liter of medium.


Supplement with 2.2 g/L sodium bicarbonate.

Storage Class Code

11 - Combustible Solids



Certificate of Analysis

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Certificate of Origin

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Quotes and Ordering

Yu Wang et al.
PloS one, 8(7), e68744-e68744 (2013-08-08)
MicroRNA-224 (miR-224) is frequently over-expressed in liver and colorectal cancers. We and others have previously described the role of miR-224 over-expression in cell proliferation in vitro but we have yet to identify the relevant miR-224 direct target. In this study
Yan Zhang et al.
Oncotarget, 8(53), 91134-91149 (2017-12-07)
Human osteosarcoma is considered a malignant tumor with poor prognosis that readily metastasizes. Tetrahydrocurcumin (THC) has been reported to have anti-tumor activity in numerous tumors. In addition, hypoxia-inducible factor-1α (HIF-1α) has been demonstrated to be associated with tumor metastasis by
Ying Liu et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 44(4), 1396-1410 (2017-12-05)
Osteosarcoma (OS) is the most common primary malignant tumor of bone tissue. Although treatment effectiveness has improved, the OS survival rate has fluctuated in recent years. Andrographolide (AG) has been reported to have antitumor activity against a variety of tumors.
Xiang-Ping Liu et al.
Journal of experimental & clinical cancer research : CR, 28, 52-52 (2009-04-21)
RhoA and RhoC are deregulated by over expression in many human tumors, including colorectal cancer. Some reports show that they play a pivotal role in the carcinogenesis, tumor development and infiltration metastasis. In this study, for the first time we
Archana Kumari et al.
The EMBO journal, 39(14), e104006-e104006 (2020-06-23)
Cellular studies of filamentous actin (F-actin) processes commonly utilize fluorescent versions of toxins, peptides, and proteins that bind actin. While the choice of these markers has been largely based on availability and ease, there is a severe dearth of structural


McCoy's 5A Modified Media Formulation

McCoy's 5A Modified Media Formulation

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Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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