Merck
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M5310

Sigma-Aldrich

Mineral oil

light oil, suitable for mouse embryo cell culture, BioXtra

CAS Number:
EC Number:
MDL number:
NACRES:
NA.75

Quality Level

sterility

sterile-filtered

product line

BioXtra

form

light oil

storage condition

protect from light

technique(s)

cell culture | embryo: suitable

refractive index

n20/D 1.467 (lit.)

density

0.82-0.88 g/mL at 25 °C

format

neat

InChI key

AEOVEGJBKQQFOP-DDVLFWKVSA-L

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General description

Mineral oil or liquid petroleum is used for conservation of various types of cutltures. The advantages of using mineral oil are that the growth rate is decreased and the evaporation of water from the medium is almost completely eliminated. Thus, sub-culturing is reduced leading to increase in the viability of the culture. The first time mineral oil was used was in 1914, and it was used to overlay bacterial culture.

Application

Mineral oil has been commonly used and recommended for overlaying mouse embryo cultures to prevent drying during the procedure.
Mineral oil is used for the following applications:
  • Used for cell viability studies in preparing the culture media or media supplements
  • Used tp protect array from evaporation by adding mineral oil around the array
  • Used to prepare the needle for microinjection (microinjection needle pulling, sharpening, and loading)
  • Used in PCR setup

Features and Benefits

Ready-to-use sterile filtered product conveniently packaged in PET bottles.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

No data available

Flash Point(C)

No data available

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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