Merck
All Photos(2)

M5670

Sigma-Aldrich

Anti-MAP Kinase (ERK-1, ERK-2) antibody produced in rabbit

whole antiserum

Synonym(s):
Anti-Mitogen Activated Protein Kinase, Anti-MAP Kinase (ERK-1, ERK-2)
MDL number:
NACRES:
NA.44

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

mol wt

antigen ERK-2 42 kDa
antigen, ERK-1 44 kDa

contains

15 mM sodium azide

species reactivity

mouse, rat, human

packaging

antibody small pack of 25 μL

technique(s)

microarray: suitable
western blot: 1:40,000-1:20,000 using rat brain extract and mouse NIH 3T3 fibroblast extract, respectively

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

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General description

MAP kinase (MAPK, mitogen-activated protein kinase) is also termed as extracellular regulated protein kinase (ERK). It consists of a family of protein kinases. MAP kinase isoforms are widely expressed in the central nervous system, thymus, spleen, heart, lung, kidney, and in high levels in PC12 cells and in fibroblasts.

Specificity

Staining of the 42 kDa and 44 kDa bands is specifically inhibited with MAP kinase peptide (317-339), but not with MAP kinase kinase (MEK) peptide (34-48) corresponding to N-terminal sequence of MEK. Anti-MAPK Kinase (ERK-1, ERK-2) specifically reacts with both phosphorylated and unphosphorylated forms of ERK-1 and ERK-2 (44 and 42 kDa, respectively).

Immunogen

synthetic peptide corresponding to amino acids 317-339 derived from subdomain XI of human MAP kinase (ERK1).

Application

Anti-MAP Kinase (ERK-1, ERK-2) antibody produced in rabbit has been used in western blot analysis at a minimum dilution of 1:40000 in rat brain extract and at 1:20000 in mouse NIH 3T3 fibroblasts.

Biochem/physiol Actions

Mitogen-activated protein kinase (MAPK) superfamily of enzymes is involved in widespread signalling pathways. Members of this family include the ERK1/2 (extracellular signal-regulated protein kinase, also termed p42/p44 MAPK), JNK and p38 MAPK subfamilies. These are the terminal enzymes in a signalling cascade where each kinase phosphorylates and activates the next member in the sequence. Phosphorylation of both tyrosine and threonine is essential for the full activation of all MAPKs. Several kinases participate in activation of the ERK cascade. This cascade is initiated by the small G protein Ras, which upon stimulation causes activation Raf1 kinase. Raf1 continues the transmission by activating MEK. Activated MEK appears to be the only kinase capable of specifically phosphorylating and activating ERK. ERK appears to be an important regulatory molecule, which by can phosphorylate regulatory targets in the cytosol (phospholipase A2, PLA2), translocated into and phosphorylate substrates in the nucleus (ELK1). The activation of ERK cascade mediates and regulates the signal transduction pathways in response to stress, mitogenic signals and is important in development and differentiation, learning, memory and survival. MAPK plays a crucial role in various signal transduction pathways, leading signals of growth factor and G protein coupled receptors to their intracellular targets. MAP kinase regulates several cellular processes including proliferation, differentiatio, cellular morphology and oncogenesis.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

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Quotes and Ordering

ErbB3/HER3 does not homodimerize upon neuregulin binding at the cell surface
Berger MB, et al.
Febs Letters, 569(1-3), 332-336 (2004)
Effect of zinc on high glucose-induced epithelial-to-mesenchymal transition in renal tubular epithelial cells
Zhang X, et al.
International Journal of Molecular Medicine, 35(6), 1747-1754 (2015)
GSK3 inhibitors regulate MYCN mRNA levels and reduce neuroblastoma cell viability through multiple mechanisms, including p53 and Wnt signaling
Duffy DJ, et al.
Molecular Cancer Therapeutics, 13(2), 454-467 (2014)
T T Giang Ho et al.
The Journal of biological chemistry, 283(31), 21588-21598 (2008-06-06)
RhoA plays a significant role in actin stress fibers formation. However, silencing RhoA alone or RhoA and RhoC did not completely suppress the stress fibers suggesting a residual "Rho-like" activity. RhoB, the third member of the Rho subclass, is a
Mitogen-Activated Protein Kinases and Their Role in Radiation Response.
Anupama M and Rajagopal R
Genes & Cancer, 4(9-10), 401-408 (2013)

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