Merck
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M7802

Sigma-Aldrich

Monoclonal Anti-MAP Kinase, Activated/monophosphorylated (Phosphothreonine ERK-1&2) antibody produced in mouse

~2 mg/mL, clone ERK-PT115, purified immunoglobulin, buffered aqueous solution

Synonym(s):
Anti-ERK, Anti-ERK-2, Anti-ERK2, Anti-ERT1, Anti-MAPK2, Anti-NS13, Anti-P42MAPK, Anti-PRKM1, Anti-PRKM2, Anti-p38, Anti-p40, Anti-p41, Anti-p41mapk, Anti-p42-MAPK
MDL number:
NACRES:
NA.44

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

ERK-PT115, monoclonal

form

buffered aqueous solution

mol wt

antigen, ERK-1 44 kDa
antigen, ERK-2 42 kDa

species reactivity

human, rat

concentration

~2 mg/mL

technique(s)

capture ELISA: suitable
immunocytochemistry: suitable
microarray: suitable
western blot: 0.5 μg/mL using extract of a rat fibroblast cell line, Rat1, activated with sorbitol

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

General description

Activated/ Monophosphorylated (Phosphothreonine ERK-1&2) (mouse IgG1 isotype) is derived from the ERK-PT115 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Mitogen-activated protein kinase 1 (MAPK1) is mapped to human chromosome 22q11.22 and MAPK3 is present on human chromosome 16p11.2.

Specificity

The antibody reacts specifically with the monophosphorylated threonine and the active diphosphorylated forms of MAP kinase (ERK-1 and ERK-2). It does not recognize the non-phosphorylated form of the MAP kinase molecule, or the diphosphorylated forms of JNK and p38 MAPK. The epitope recognized by the antibody contains the phosphorylated threonine residue within the regulatory site of active MAP kinase (e.g.,Thr183 in ERK-2).

Immunogen

synthetic peptide HTGFLpTEYVAT, corresponding to the phosphorylated form of ERK-activation loop.

Application

Monoclonal Anti-MAP Kinase, Activated/monophosphorylated (Phosphothreonine ERK-1&2) antibody produced in mouse has been used in:
  • immunoblotting
  • western blotting
  • immunofluorescence
  • enzyme linked immunosorbent assay (ELISA)
  • immunochemistry

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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