This product is dissolved in a TE buffer consisting of 10 mM Tris-HCl with 1 mM EDTA.
Pricing and availability is not currently available.
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form
liquid
concentration
50 ng/μL
color
colorless
storage temp.
−20°C
Related Categories
General description
Fully methylated Jurkat DNA. 5-methyl-cytidine (5mC) content determined by mass spectrometry. See lot specific certificate of analysis or vial label for 5mC content of each lot.
Application
Methylated Control DNA has been used as a negative control in bisulfite sequencing of embryonic genomic DNA.[1]
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
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Non-CpG methylation by DNMT3B facilitates REST binding and gene silencing in developing mouse hearts
Zhang D, et al.
Nucleic Acids Research, 45(6), 3102-3115 (2016)
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What buffer is the Methylated Control DNA dissolved in?
1 answer-
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It has been written 50 ng/ul for concentration. What is the total volume (as ul or ml) and total probe amount (as ug or mg) in the vial. Could you inform me, please
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The total volume is 16ul.
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What is the percentage of 5-Methylcytosine?
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As mentioned on the product detail page, the % 5mC content is reported in the lot specific Certificate of Analysis, as well as the product label. The specification for 5mC is >/= 4.5%. The historical average is approximately 6.3%. Please see the sample Certificate of Analysis: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/M8570-1VLSLCK7031.pdf
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