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MAK038

Sigma-Aldrich

NADP/NADPH Quantitation Kit

sufficient for 100 colorimetric tests

Synonym(s):
NADP/NADPH Assay Kit
eCl@ss:
32161000
NACRES:
NA.25

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

shipped in

dry ice

storage temp.

−70°C

General description

Nicotinamide adenine dinucleotide phosphate (NADP) is an enzymatic cofactor involved in many redox reactions where it cycles between the reduced (NADPH) and oxidized (NADP) forms. NADP is also involved in biosynthetic reactions such as lipid and nucleic acid synthesis where it functions as a reducing agent. The oxidative branch of the Pentose Phosphate Pathway (PPP) is the major source of NADPH produced in animal cells.

Application

NADP/NADPH (nicotinamide adenine dinucleotide phosphate) Quantitation Kit has been used for quantification of NADP/NADPH. It has been used to quantify NAD(P)H in order to determine SHMT2 (serine hydroxymethyltransferase 2) specific enzyme activity.

Suitability

Suitable for the detection of NADP, NADPH and their ratio in a variety of biological samples including cell and tissue lysates

Principle

The NADP/NADPH Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides, NADP (nicotinamide adenine dinucleotide phosphate) and NADPH, and their ratio. There is no requirement to purify NADP/NADPH from samples. This assay is specific for NADP and NADPH and does not detect NAD or NADH. NADPt (NADP and NADPH) or NADPH are quantified in a colorimetric assay (450 nm).

Pictograms

CorrosionHealth hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Certificate of Analysis

Certificate of Origin

Luigi Mele et al.
Cell death & disease, 9(5), 572-572 (2018-05-16)
Pentose phosphate pathway (PPP) is a major glucose metabolism pathway, which has a fundamental role in cancer growth and metastasis. Even though PPP blockade has been pointed out as a very promising strategy against cancer, effective anti-PPP agents are not
Andreas Carstensen et al.
Plant physiology, 177(1), 271-284 (2018-03-16)
Phosphorus (P) is an essential macronutrient, and P deficiency limits plant productivity. Recent work showed that P deficiency affects electron transport to photosystem I (PSI), but the underlying mechanisms are unknown. Here, we present a comprehensive biological model describing how
Mechanisms of action of isoniazid.
Timmins G S and Vojo D
Molecular Microbiology, 62(5), 1220-1227 (2006)
Q Shi et al.
Toxicology, 381, 64-74 (2017-02-28)
It has been well established that inflammation and concurrent mutagenic exposures drive the carcinogenic process in a synergistic way. To elucidate the role of the inflammatory cytokine IL-8 in this process, we studied its effect on the activation and deactivation
Metabolic engineering of Escherichia coli: increase of NADH availability by overexpressing an NAD+-dependent formate dehydrogenase.
Berri'os-Rivera S J, et al.
Metabolic engineering, 4(3), 217-229 (2002)

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