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Merck

MAK117

Glycerol Assay Kit

sufficient for 200 colorimetric or fluorometric tests

Synonym(s):

Glycerin Assay Kit, Glycerine Assay Kit

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NACRES:
NA.84
UNSPSC Code:
12161503

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Product Name

Glycerol Assay Kit, sufficient for 200 colorimetric or fluorometric tests

usage

sufficient for 200 colorimetric or fluorometric tests

species reactivity

Drosophila, human, mouse

packaging

pkg of 1 kit

storage condition

dry at room temperature

technique(s)

activity assay: suitable

accession no.

NM_000167.6
NM_001273184.2
P13706
P32189

application(s)

cosmetics
environmental
food and beverages

detection method

colorimetric
fluorometric

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−20°C

Gene Information

Drosophila ... Gpdh1(33824)
human ... GK(2710 )
mouse ... Gpd1(14555)

input

food(s)
beverage(s)
biological sample(s)

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Glycerol Assay Kit sufficient for 200 colorimetric or fluorometric tests

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detection method

colorimetric, fluorometric

detection method

colorimetric, fluorometric

detection method

colorimetric, fluorometric

detection method

colorimetric

usage

sufficient for 200 colorimetric or fluorometric tests

usage

sufficient for 100 colorimetric or fluorometric tests

usage

sufficient for 100 colorimetric or fluorometric tests

usage

sufficient for 180 colorimetric tests

shipped in

dry ice

shipped in

dry ice

shipped in

-

shipped in

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

application(s)

cosmetics
environmental
food and beverages

application(s)

pharmaceutical

application(s)

-

application(s)

-

relevant disease(s)

cancer

relevant disease(s)

-

relevant disease(s)

cancer; cardiovascular diseases; hematological disorder

relevant disease(s)

hematological disorder; gastrointestinal diseases

Application

Glycerol assay kit has been used to quantify glycerol release from mouse 3T3-L1 cell culture supernatant samples[1] and to measure plasma glycerol levels.[2]
This kit is suitable for glycerol detection in foods, beverages, pharmaceutical formulations, and biological samples.

Biochem/physiol Actions

In this kit, glycerol concentration is determined by a coupled enzyme assay involving glycerol kinase and glycerol phosphate oxidase, resulting in a colorimetric (570 nm)/fluorometric (lex = 535/lem = 587 nm) product, proportional to the glycerol present. The linear range of detection for this kit is 10-1,000 μM (colorimetric) and 2-50 μM (fluorometric).

Features and Benefits

Compatible with high-throughput handling systems.

General description

Glycerol, also referred to as glycerin or glycerine, is a 3-carbon sugar alcohol that forms the backbone of fatty acids. It is also a component of phospholipids and triglycerides. Glycerol is a sweet-tasting, hygroscopic liquid. Glycerol is widely used in food, beverage, and pharmaceutical formulations[3] and is the main waste by-product of biodiesel production via transesterification.

Storage Class

10 - Combustible liquids

wgk

WGK 2

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
117CF08A22
117CF08A22
117CF07A09
117CF07A09
117CF04A04

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Martina Rudnicki et al.
Frontiers in physiology, 9, 1452-1452 (2018-11-09)
Background: Impaired capillary growth (angiogenesis) in skeletal muscle and adipose tissue contributes to the development of metabolic disorders in obese males. This association remains unexplored in females, despite mounting evidence that endothelial cells have sex-specific transcriptional profiles. Therefore, herein we
Nicole E Nozzi et al.
ACS synthetic biology, 6(11), 2136-2144 (2017-07-19)
Cyanobacteria have attracted significant interest as a platform for renewable production of fuel and feedstock chemicals from abundant atmospheric carbon dioxide by way of photosynthesis. While great strides have been made in developing this technology in freshwater cyanobacteria, logistical issues
Jessica C Brooks et al.
The Analyst, 141(20), 5714-5721 (2016-08-04)
Employing 3D-printed templates for macro-to-micro interfacing, a passively operated polydimethysiloxane (PDMS) microfluidic device was designed for time-resolved secretion sampling from primary murine islets and epidiymal white adipose tissue explants. Interfacing in similar devices is typically accomplished through manually punched or
Sylvia J Gasparini et al.
Diabetologia, 62(8), 1463-1477 (2019-05-18)
Chronic glucocorticoid therapy causes insulin resistance, dyslipidaemia, abnormal fat accumulation, loss of muscle mass and osteoporosis. Here we describe a hitherto unknown sexual dimorphism in the metabolic response to chronic glucocorticoid exposure in mice. This led us to investigate whether
Paula Dunayevich et al.
Scientific reports, 8(1), 15168-15168 (2018-10-13)
Cells make decisions based on a combination of external and internal signals. In yeast, the high osmolarity response (HOG) is a mitogen-activated protein kinase (MAPK) pathway that responds to a variety of stimuli, and it is central to the general
View All Related Papers

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Questions

1–9 of 9 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. Is this assay (MAK117) and the free fatty acid assay kit (MAK466) suitable for use with serum or supernatant samples which have been frozen prior to use?

    1 answer

    1. Yes, the serum or supernatant samples can be stored frozen. The glycerol analyzed by the MAK117 kit and the fatty acids analyzed by the MAK466 kit would not be harmed or their concentration changed by freezing samples. In fact, if samples need to be stored, they would logically be more stable if kept frozen.

      Helpful?

  3. Does the presence of ethylene glycole, methanol or propanediols affect the analysis?

    1 answer

    1. There is no information regarding possible interference of Ethylene glycol, Methanol, or Propanediols with the assay kit, but such interferences are not likely. The use of Glycerol kinase makes the kit specific to the reaction of Glycerol with ATP.

      Helpful?

  4. I'd like know what is the maximum concentration of glycerol measured by the instrument? How precise is the analysis measurement (standard deviation expected)? Can it be used for a water solution at 5% glycerol in weight (glycerol 99% pure)?

    1 answer

    1. Linear detection range in 96-well plate: 10 to 1000 µM (92 µg/dL to 9.2 mg/dL) glycerol for colorimetric assays and 2 to 50 µM for fluorimetric (1.84 µg/dL - 46 µg/dL) assays. A 5% glycerol solution with a density of approximately 1.01 g/mL translates to a solution with a concentration of 0.548 M or 548 mM or 548,000 µM. The sample would have to be diluted at least 1000x before it falls into the middle of the linear detection range for the colorimetric assay. Please dilute the sample in water and account for the dilution in the glycerol concentration calculation. No specific upper limit of quantitation or standard deviation has been assigned using this kit for this sample.

      Helpful?

  5. After reading the description of this product, I can't help but wonder what the contents of the kit are, and if there is any additional documentation related (protocol example, enzyme description, standard concentrations and curves).

    1 answer

    1. Full product information, including the protocol, is available in the product datasheet. The formulation of the components is considered proprietary, with the exception of the standard - 100mM Glycerol. This is an enzymatic assay that utilizes glycerol kinase and glycerol phosphate oxidase. Please see the link below to review additional details:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/260/409/mak117bul.pdf

      Helpful?

  6. Is there any information available on how to prepare tissue for assay, considering that technical bulletin MAK117 does not include any recommendations on this matter?

    1 answer

    1. The general recommendation for tissue sample preparation is as follows:
      1. Begin with 20-100 mg tissue and add 200-1000 uL ice-cold PBS.
      2. Lysis can be achieved by homogenization (10-20 passes in a Dounce homogenizer on ice) or by sonication (preferably performed in an ice-water bath). The degree of tissue lysis can be checked under a microscope.
      3. Centrifuge the homogenate at 14,000 g for 10 min.
      4. Transfer the clear supernatant into a clean tube and use it for the assay.

      Helpful?

  7. Can serum glycerol content be analyzed or just plasma?

    1 answer

    1. Yes, this product can determine glycerol content in serum samples.

      Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/260/409/mak117bul.pdf

      Helpful?

  8. How long are reagents stable at 2-8 C after initial thaw?

    1 answer

    1. The Assay Buffer, MAK117A, may be stored at 2–8 Celsius. For all other components, it is recommended to aliquot and freeze at - 20 deg C. Avoid repleated freeze/thaw cycles.

      Helpful?

  9. How is shipping temperature determined? And how is it related to the product storage temperature?

    0 answers

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