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Merck

MAK144

Fluorometric Intracellular Ros Kit

sufficient for 200 fluorometric tests (orange)

Synonym(s):

Fluorometric ROS Assay Kit

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About This Item

EC Number:
200-664-3
UNSPSC Code:
12161503
NACRES:
NA.25

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usage

sufficient for 200 fluorometric tests (orange)

application(s)

cosmetics
food and beverages

detection method

fluorometric

relevant disease(s)

cancer; neurological disorders

storage temp.

−20°C

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Fluorometric Intracellular Ros Kit sufficient for 200 fluorometric tests (orange)

Sigma-Aldrich

MAK144

Fluorometric Intracellular Ros Kit

Fluorometric Intracellular Ros Kit sufficient for 200 fluorometric tests (Deep red)

Sigma-Aldrich

MAK142

Fluorometric Intracellular Ros Kit

Fluorometric Intracellular Ros Kit sufficient for 200 fluorometric tests (red)

Sigma-Aldrich

MAK145

Fluorometric Intracellular Ros Kit

Fluorimetric Hydrogen Peroxide Assay Kit sufficient for 500 fluorometric tests (red fluorescence)

Sigma-Aldrich

MAK165

Fluorimetric Hydrogen Peroxide Assay Kit

detection method

fluorometric

detection method

fluorometric

detection method

fluorometric

detection method

fluorometric

usage

sufficient for 200 fluorometric tests (orange)

usage

sufficient for 200 fluorometric tests (Deep red)

usage

sufficient for 200 fluorometric tests (red)

usage

sufficient for 500 fluorometric tests (red fluorescence)

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

application(s)

cosmetics
food and beverages

application(s)

cosmetics
food and beverages

application(s)

-

application(s)

-

relevant disease(s)

cancer; neurological disorders

relevant disease(s)

neurological disorders; cancer

relevant disease(s)

neurological disorders; cancer

relevant disease(s)

cardiovascular diseases; aging/geriatric diseases; orthopedic diseases; pulmonary disorders; neurological disorders

General description

Reactive oxygen species (ROS) are generated as a result of the reduction of oxygen during aerobic respiration and by various enzymatic systems within the cell.[1] At physiological levels, ROS contribute to cell signaling and host defense.[2] Increased ROS generation above the biological system′s detoxification capacity results in oxidative stress and cellular damage. The main damage to cells results from the ROS-induced alteration of macromolecules such as polyunsaturated fatty acids in membrane lipids, essential proteins, and DNA.[1][2] ROS has been implicated in disease states, such as Alzheimer′s disease, Parkinson′s disease, cancer, and aging.[3]

Application

Fluorometric intracellular reactive oxygen species (ROS) Kit has been used to evaluate the mitochondrial damage by mechanical ventilation (MV).[4] It is also used to measure the production of intracellular ROS.[5]
This kit is suitable for the detection of intracellular ROS by fluorescence microplate reader, flow cytometer, or a fluorescent microscope with TRITC filter.

Biochem/physiol Actions

The Fluorometric Intracellular ROS Assay Kit provides a sensitive, one-step fluorometric assay to detect intracellular ROS (especially superoxide and hydroxyl radical) in live cells within 1 hour incubation. ROS react with a cell-permeable sensor, resulting in a fluorometric product (λex = 540/λem = 570 nm) proportional to the amount of ROS present. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
3511771
3321203
3511771
3321203
318029

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Udayakumar Karunakaran et al.
Redox biology, 45, 102029-102029 (2021-06-10)
Prolonged hyperglycemia plays a major role in the progression of β-cell loss in diabetes mellitus. Here we report an insulin sensitizer thiazolidinedione Pioglitazone selectively preserves the beta cells against high glucose-induced dysfunction by activation of AMPK and Glutaminase 1 (GLS1)
Oxidized low density lipoprotein (ox-LDL) binding to ox-LDL receptor-1 in endothelial cells induces the activation of NF-KB through an increased production of intracellular reactive oxygen species.
Cominacini L, et al.
The Journal of Biological Chemistry, 275(17), 12633-12638 (2000)
High tidal volume induces mitochondria damage and releases mitochondrial DNA to aggravate the ventilator-induced lung injury.
Lin J, et al.
Frontiers in Immunology, 9, 1477-1477 (2018)
Tao Zheng et al.
Frontiers in pharmacology, 12, 669146-669146 (2021-05-25)
Although miR-327 had a protective effect on cardiomyocytes as described previously, the potential mechanism still needs further exploration. The aim of this study was to investigate the role and mechanism of miR-327 on oxidative stress in myocardial ischemia/reperfusion injury (MI/RI)
Neurodegenerative diseases and oxidative stress.
Barnham K J, et al.
Nature Reviews. Drug Discovery, 3(3), 205-205 (2004)
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Questions

1–3 of 3 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. Does it work for non-cellular environments, like a solution? Thanks.

    1 answer

    1. This kit is not suitable for use in non-cellular environments and is intended only for the analysis of live cells as described in the Technical Bulletin.

      Helpful?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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