Merck
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MAK193

Sigma-Aldrich

Citrate Synthase Activity Assay Kit

Sufficient for 100 Colorimetric tests

EC Number:
NACRES:
NA.84

detection method

colorimetric

storage temp.

−20°C

Gene Information

human ... CS(1431)
mouse ... CS(12974)
rat ... CS(170587)

Related Categories

General description

Citrate Synthase (CS; EC 2.3.3.1) is a mitochondrial enzyme found in all cells capable of oxidative metabolism. It catalyzes the synthesis of citrate from oxaloacetate in the Krebs tricarboxylic acid cycle.1,2 CS is involved in energy production, lipogenesis, and cholesterologenesis, and its activity follows a circadian pattern.

Application

Citrate Synthase Activity Assay Kit has been used to measure the activity of the enzyme citrate synthase in samples.

Suitability

Suitable for the detection of citrate synthase activity in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.

Principle

The Citrate Synthase Activity Assay kit provides a simple and direct procedure for measuring the activity of CS in a variety of tissues, cell cultures (adherent or suspension), and purified mitochondria. CS activity is determined using a coupled enzyme reaction, which results in a colorimetric (412 nm) product proportional to the enzymatic activity present. One unit of CS is the amount of enzyme that generates 1.0 mmole CoA per minute at 25 °C and pH 7.2.

Kit Components Only

Product No.
Description

  • CS Assay Buffer

  • CS Substrate Mix

  • CS Developer

  • GSH Standard, Reduced

  • CS Positive Control

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

10 - Combustible liquids

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Product Information Sheet

Quotes and Ordering

Tony Chao et al.
American journal of physiology. Cell physiology, 317(6), C1229-C1238 (2019-09-19)
Severe burn injury results in systemic disruption of metabolic regulations and impaired cardiac function. Restoration of hemodynamic homeostasis utilizing intravenous (IV) fluids is critical for acute care of the burn victim. However, the effects of burns and resuscitation on cardiomyocyte
Tomoaki Aoki et al.
Frontiers in medicine, 8, 666735-666735 (2021-04-30)
Background: Identification of the mechanisms underlying mitochondrial dysfunction is key to understanding the pathophysiology of acute injuries such as cardiac arrest (CA); however, effective methods for measurement of mitochondrial function associated with mitochondrial isolation have been debated for a long
P Dongil et al.
Scientific reports, 8(1), 13810-13810 (2018-09-16)
Metabolic dysfunction in the liver is the cause of numerous pathologies, which are associated with an altered redox state. PASK (PAS Domain Kinase) is a nutrient and bioenergetic sensor. We contend that PASK could act as an oxidative stress sensor
Regulation of Expression of Citrate Synthase by the Retinoic Acid Receptor-Related Orphan Receptor α (RORα)
Crumbley C, et al.,
PLoS ONE, 7, e33804 -e33804 (2012)
Xi Lin et al.
International journal of molecular sciences, 21(3) (2020-01-26)
Maintaining an active fatty acid metabolism is important for renal growth, development, and health. We evaluated the effects of anaplerotic and ketogenic energy sources on fatty acid oxidation during stimulation with clofibrate, a pharmacologic peroxisome proliferator-activated receptor α (PPARα) agonist.

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