Merck
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MAK339

Sigma-Aldrich

Lipopolysaccharide (LPS) Isolation Kit

sufficient for 10 isolation(s)

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usage

sufficient for 10 isolation(s)

application(s)

sample preservation

storage temp.

−20°C

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This Item
L6261L6529L2630
storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

application(s)

sample preservation

application(s)

-

application(s)

-

application(s)

cell analysis
clinical research
life science and biopharma

General description

The outer membrane of gram-negative bacteria contains lipopolysaccharide (LPS), a low molecular weight carbohydrate with a molecular mass of 10-20 kDa. It is heterogeneous and composed of O antigen (a repeating glycan polymer), core oligosaccharide (which links the O antigen to Lipid A - the third component, and non-carbohydrate components such as phosphate and amino acids groups. Lipid A, has multiple fatty acids which serve to anchor LPS into the bacterial membrane allowing the O antigen and core oligosaccharide to protrude and contributes to a major part of the toxicity of gram-negative bacteria. Also known as endotoxin, when consumed by animals, LPS induces a strong inflammatory response and/or sepsis. 

Suitability

Suitable for the isolation of Lipopolysaccharides from outer membrane of gram negative bacteria.

Principle

The Lipopolysaccharide (LPS) Isolation Kit uses bacterial membrane lysis buffer and protein digestion to yield micrograms of LPS from bacterial culture (approximately 1-4% of dry weight). This kit does not use chloroform or phenol like traditional methods, and it will yield pure LPS in less than 2 hours that can be easily characterized and quantified.

Pictograms

CorrosionHealth hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1 - Resp. Sens. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Zhiyuan He et al.
Frontiers in nutrition, 9, 864080-864080 (2022-04-12)
Calf diarrhea induced by enteroaggregative E. coli (EAEC) spreads fast among young ruminants, causing continuous hazard to dairy industry. Antimicrobial drug abuse aggravates the incidence rate of multi-drug resistant (MDR) extended-spectrum β-lactamase-producing E. coli (ESBL-EC). However, knowledge of detection and
Zhiyuan He et al.
Microbiome, 10(1), 79-79 (2022-06-02)
Antimicrobials are often used to prevent and treat diarrhea induced by enteroaggregative Escherichia coli (EAEC) in young ruminants. However, drug overuse or misuse accelerates the spread of multidrug-resistant extended-spectrum β-lactamase (ESBL)-producing E. coli. Thus, supplementary foods as alternatives to antibiotics

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