MAK453

Glutathione S-transferase Assay Kit

Sufficient for 100 colorimetric tests

Properties

• Quality Level: 100
• detection method: colorimetric
• relevant disease(s): neurological disorders, asthma; pulmonary disorders, diabetes; cancer; endocrinological disorders
• storage temp.: −20°C

Description

General description

Glutathione transferase (GST) is a multifunctional enzyme that plays an important role in cellular detoxification. GST protects cells against foreign compounds such as carcinogens and drugs by catalyzing the attachment of glutathione to the compounds electrophilic and/or hydrophobic sites.

Features and Benefits

• Fast and sensitive. Linear detection range (20 μL sample): 2 to 80 U/L for 10 min reaction at 25 °C.
• Compatible with high-throughput handling systems.

Suitability

The kit is suitable for Glutathione S-transferase activity determination in cell lysates, tissues, etc.

Principle

The Glutathione S-transferase Assay Kit is based on the GST enzyme reaction between GSH and the GST substrate, CDNB (1- chloro-2,4-dinitrobenzene). The GST catalyzed formation of GS-DNB produces a dinitrophenyl thioether which can be detected spectrophotometrically at A₃₄₀ nm. The rate of increase in absorbance at A₃₄₀ nm is directly proportional to the GST activity in the sample.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317 - H319 - H412
• Precautionary Statements: P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Aquatic Chronic 3 - Eye Irrit. 2 - Skin Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): 188.6 °F
• Flash Point(C): 87 °C