The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.
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Choroform extraction should not interfere with the protocol as long as the DNA was not eluted with the chloroform.
No, you can use as many wells as needed. You will want to ensure that all the wells that are not being used are covered well with Parafilm, Saran Wrap or some other plate sealer.
The detection limit of this kit is 5 nanograms of fully methylated DNA.
No. A fresh dilution of the antibody should be prepared each time the kit is used. Diluting the antibody all at once will decrease its stability and it's effectiveness in the kit.
The antibody works on 5-me-C, so any species with DNA that is methylated at the 5 position of cytidine residues will work.
Yes, Product MDQ1, Imprint® Methylated DNA Quantification Kit, is shipped in 2 parts. Part 1 contains, 2-8 °C components (W4267, D8568, C3493, D8818, D8693, S3447, and A4231) and Part 2 contains -20 °C components (M8570 and B6561).
Ask a Scientist here.
The overall degree of methylation of a genome can be a useful measure of global regulatory changes. Measurement of this parameter is usually performed after complete digestion to the single base and then analyzed using HPLC or mass spectrophotometry. The Global DNA Modification Kit (MDQ1), allows the researcher to monitor DNA methylation using a format similar to a sandwich ELISA assay.