Yes. However, if you are currently required to use an endofree plasmid purification kit, we recommend using the GenElute HP Endotoxin-free Kit.
NA0310
GenElute™ HP Plasmid Maxiprep Kit
sufficient for 25 purifications
Synonym(s):
GenElute™ HP Plasmid Maxiprep Kit, plasmid extraction kit, plasmid isolation kit, plasmid purification kit
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About This Item
NACRES:
NA.52
UNSPSC Code:
41105501
Pricing and availability is not currently available.
usage
sufficient for 25 purifications
Quality Level
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technique(s)
DNA purification: suitable
test parameters
sample volume: 150 mL bacterial culture
greener alternative category
storage temp.
15-25°C
General description
The GenElute HP Plasmid Maxiprep Kit offers a simple, rapid, and cost effective method for isolating plasmid DNA from recombinant E. coli cultures. The kit features a filter syringe for the rapid clearing of lysate and a silica binding column designed for either a vacuum or a spin format. Up to 1.2 mg of plasmid DNA can be isolated from a 150 mL overnight culture grown in Luria Broth (LB) medium. Note that the actual yield depends on the strain, the plasmid, and the culture medium used.
An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto a silica membrane in the presence of high salts. Contaminants are removed by two wash steps. Finally, the bound DNA is eluted in Elution Solution (Tris-HCl) or water.
The recovered plasmid DNA is predominately in its supercoiled form. The DNA is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, transformation, and transfection.
An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto a silica membrane in the presence of high salts. Contaminants are removed by two wash steps. Finally, the bound DNA is eluted in Elution Solution (Tris-HCl) or water.
The recovered plasmid DNA is predominately in its supercoiled form. The DNA is ready for immediate use in downstream applications such as restriction digestion, ligation, sequencing, PCR, transformation, and transfection.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
Application
GenElute™ HP Plasmid Maxiprep Kit has been used in the isolation of plasmids.
Features and Benefits
- From harvested bacterial culture to pure plasmid DNA in 30 minutes or less
- Up to 1.2 mg of high-copy plasmid DNA
- Flexibility of a vacuum or spin format
- Contains fewer plastic components than other high speed kits, reducing the amount of waste
- No phenol/chloroform extraction or alcohol precipitation required
Other Notes
For additional information, please see www.sigma-aldrich.com/genelutehp.
Legal Information
GenElute is a trademark of Sigma-Aldrich Co. LLC
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This Item | |||
|---|---|---|---|
| usage sufficient for 25 purifications | usage sufficient for 10 purifications | usage sufficient for 15 purifications | usage sufficient for 15 purifications |
| technique(s) DNA purification: suitable | technique(s) DNA purification: suitable | technique(s) DNA purification: suitable | technique(s) DNA purification: suitable |
| test parameters sample volume: 150 mL bacterial culture | test parameters sample volume: 50 mL bacterial culture | test parameters : <2 hr kit run time for up to 12 preps, sample volume: 25-200 mL bacterial culture | test parameters : ~45 min kit run time, sample volume: 25-200 mL bacterial culture |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| greener alternative category | greener alternative category | greener alternative category , Aligned | greener alternative category |
| greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics Designing Safer Chemicals | greener alternative product characteristics Inherently Safer Chemistry for Accident Prevention | greener alternative product characteristics Designing Safer Chemicals |
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signalword
Danger
target_organs
Central nervous system
Storage Class
3 - Flammable liquids
flash_point_f
77.0 °F - closed cup
flash_point_c
25 °C - closed cup
wgk
WGK 3
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Corr. 1B - STOT SE 3
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Protocols
Follow this simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures with the GenElute™ HP Plasmid Maxiprep kit.
Fraser Soares et al.
Blood, 137(16), 2171-2181 (2020-12-04)
Acute myeloid leukemia (AML) remains a devastating disease in need of new therapies to improve patient survival. Targeted adoptive T-cell therapies have achieved impressive clinical outcomes in some B-cell leukemias and lymphomas but not in AML. Double-negative T cells (DNTs)
Musaddeque Ahmed et al.
Nature communications, 12(1), 1781-1781 (2021-03-21)
Prostate cancer (PCa) risk-associated SNPs are enriched in noncoding cis-regulatory elements (rCREs), yet their modi operandi and clinical impact remain elusive. Here, we perform CRISPRi screens of 260 rCREs in PCa cell lines. We find that rCREs harboring high risk
Jaeho Yoon et al.
Biomedicines, 9(9) (2021-09-29)
Renal hypouricemia is a rare genetic disorder. Hypouricemia can present as renal stones or exercise-induced acute renal failure, but most cases are asymptomatic. Our previous study showed that two recessive variants of SLC22A12 (p.Trp258*, pArg90His) were identified in 90% of
Global Trade Item Number
| SKU | GTIN |
|---|---|
| NA0310-1KT | 04061834119036 |
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Is plasmid DNA isolated with this kit suitable for use in transfection?
1 answer-
Helpful?
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Which format gives a better yield, vacuum or spin?
1 answer-
There is no difference in plasmid recovery when using either format.
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How long does it take to isolate plasmid using the vacuum format?
1 answer-
A midiprep or maxiprep requires only 30 minutes to complete.
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How do the GenElute HP Plasmid Kits differ from other plasmid purification systems?
1 answer-
The GenElute HP Plasmid Kits are much faster than other commercially available kits. A filter syringe is used for preparing the cleared lysate, which replaces a lengthy centrifugation step. These kits also offer the option of using a vacuum or spin purification format rather than a gravity drip to bind, wash and elute the plasmid. In addition, samples are eluted in Tris buffer so there are no alcohol precipitations required.
Helpful?
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What is the expected yield of plasmid DNA that you can isolate from these kits?
1 answer-
Yields can reach as high as 350 mg for a midiprep and 1.2 mg for a maxiprep. As with any protocol, lower yields can be expected with low copy plasmids.
Helpful?
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Do the binding columns fit on other manufacturers’ manifolds?
1 answer-
Yes. The columns fit on all standard vacuum manifolds.
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