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P0001

Sigma-Aldrich

ReadyShield® Phosphatase Inhibitor Cocktail

For Serine/Threonine Protein Phosphatases and L-Isozymes of Alkaline Phosphatase

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Synonym(s):
Phosphatase inhibitor cocktail

Quality Level

200

form

ready-to-use solution

packaging

pkg of 1 mL (in amber polypropylene vial)
pkg of 5 mL (in amber polypropylene vial)

storage temp.

−20°C

Related Categories

General description

This phosphatase Inhibitor Cocktail contains inhibitors with a broad specificity for L-isozymes of alkaline phosphatases, and serine/ threonine protein phosphatases. Phosphatase Inhibitor Cocktail has been tested on extracts from various animal tissues such as human placenta and bovine liver, and on extracts of A431, CHO, and U937 cells.

This cocktail is supplied as a ready-to-use solution using a proprietary non-freezing formulation.
ReadyShield® P0001 is a non-freezing formulation that contains the same inhibitors as is the DMSO based cocktail P0044.

Features and Benefits

  • Does not freeze in storage for quick, convenient use.
  • Specific for Serine/Threonine Protein Phosphatases and L-Isozymes of Alkaline Phosphatase.
  • Suggested as 100 X solution.
  • Reclosable screw-top vials.
  • Can be stored in cooler for approx. 1 month or room temperature for a week.

Preparation Note

This cocktail is supplied as a clear solution in dimethyl sulfoxide. It is recommended to dilute the cocktail at 1 mL of cocktail per 500 mg of protein extracted from tissue or cells. Often a final concentration of 1% (v/v) is used, which will inhibit phosphatase activities found in the 27,000 x g supernatant from human placenta and bovine liver, and in the 100,000 x g supernatant from A431, CHO, and U937 extracts.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

ReadyShield is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Articles

ReadyShield Phosphatase and Protease Inhibitor Cocktail FAQ

ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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