P0982

JumpStart^™ REDTaq^® ReadyMix^™ Reaction Mix

for PCR

• Synonym(s): Hot start PCR master mix, Hot start master mix, Hot start taq master mix
• MDL number: MFCD00166026

Properties

• Quality Level: 200
• form: liquid
• usage: sufficient for 100 reactions; sufficient for 20 reactions; sufficient for 800 reactions
• feature: dNTPs included; hotstart
• concentration: 2.5 units/reaction (50 μL reaction volume)
• technique(s): PCR: suitable
• color: red
• input: purified DNA
• application(s): agriculture
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

JumpStart^™ REDTaq^® ReadyMix^™PCR Reaction Mix is a prepared solution combining the performance benefits of hot start PCR with the dual convenience of Sigma′s ReadyMix and REDTaq. The Master mix includes Sigma′s JumpStart Taq DNA Polymerase, 99% pure deoxynucleotides, buffer, and an inert red dye in a 2× optimized reaction concentrate. Add 25 μL of the ReadyMix to primers, template, and water to a final reaction volume of 50 μL. JumpStart Taq antibody in the reaction mix inactivates the Taq DNA polymerase at room temperature. During the first denaturation step of PCR the complex dissociates and polymerase activity is fully restored. The inert red dye does not effect automated sequencing, restriction enzyme digestion, ligation, or other downstream manipulations. The PCR product is easily separated from the dye by standard purification methods if desired. The hot start mechanism allows for room temperature set up, making it the ideal for high throughput applications.

Application

Jumpstart^™ REDTaq^® ReadyMix^™ Reaction Mix is used for direct DNA extraction and amplification using PCR. It has also been used for:

• qualitative multiplex PCR
• methylation-specific PCR (MSPCR)
• PCR amplification of cDNA synthesized from total RNA

Features and Benefits

• JumpStart^™ REDTaq^® DNA polymerase, an antibody inactivated hot start enzyme, is designed to minimize non-specific amplification while increasing target yield & specificity
• JumpStart Taq DNA polymerase provides superior amplification regardless of template concentration
• REDTaq JumpStart ReadyMix reduces pipetting steps and risk of contamination. The hot start mechanism allows for room temperature set up, making it the ideal for high throughput applications
• Inert red dye allows for easy verification that reagent has been mixed properly
• Other loading dyes are not required. Aliquots may be directly loaded onto an agarose gel after the reaction

Packaging

Default reaction volume is 50 μL

20RXN is packaged as 1 X 500 μL
100RXN is packaged as 1 X 2.5 mL
800RXN is packaged as 1 X 20 mL

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid precipitable DNA in 30 min. at 74 °C.

Other Notes

View more detailed information on JumpStart REDTaq and ReadyMix products at www.sigma-aldrich.com/hotstart.

For a typical PCR reaction, mix 25 μL of JumpStart^™ REDTaq^® ReadyMix^™ with 25 μL of a mixture containing template DNA, primers, and water. Reaction volume can be scaled if desired.

Legal Information

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.

JumpStart is a trademark of Sigma-Aldrich Co. LLC

REDTaq is a registered trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC