JumpStart REDTaq® ReadyMix Reaction Mix

for PCR

MDL number:

Quality Level




dNTPs included


2.5 units/reaction (50 μL reaction volume)


PCR: suitable



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shipped in

wet ice

storage temp.


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General description

JumpStart REDTaq ReadyMix PCR Reaction Mix is a prepared solution combining the performance benefits of hot start PCR with the dual convenience of Sigma′s ReadyMix and REDTaq. The mix includes Sigma′s JumpStart Taq DNA Polymerase, 99% pure deoxynucleotides, buffer, and an inert red dye in a 2× optimized reaction concentrate. Add 25 μL of the ReadyMix to primers, template, and water to a final reaction volume of 50 μL.

JumpStart Taq antibody in the reaction mix inactivates the Taq DNA polymerase at room temperature. During the first denaturation step of PCR the complex dissociates and polymerase activity is fully restored.

The red dye allows quick visual confirmation the enzyme has been added and properly mixed. After PCR, an aliquot may be loaded directly onto an agarose gel without the addition of loading buffers. The inert red dye does not effect automated sequencing, restriction enzyme digestion, ligation, or other downstream manipulations. The PCR product is easily separated from the dye by standard purification methods if desired.

Using JumpStart ReadyMix reduces pipetting steps and risk of contamination. The hot start mechanism allows for room temperature set up, making it the ideal for high throughput applications.


Jumpstart REDTaq® ReadyMix Reaction Mix is used for direct DNA extraction and amplification using PCR. It has also been used for:
  • qualitative multiplex PCR
  • methylation-specific PCR (MSPCR)
  • PCR amplification of cDNA synthesized from total RNA

Features and Benefits

  • JumpStart Taq DNA polymerase, an antibody inactivated hot start enzyme, is designed to minimize non-specific amplification while increasing target yield & specificity
  • JumpStart Taq DNA polymerase provides superior amplification regardless of template concentration
  • REDTaq JumpStart ReadyMix reduces pipetting steps and risk of contamination. The hot start mechanism allows for room temperature set up, making it the ideal for high throughput applications
  • Inert red dye allows for easy verification that reagent has been mixed properly
  • Other loading dyes not necessary. Aliquots may be directly loaded onto an agarose gel after the reaction


Default reaction volume is 50 μL

20RXN is packaged as 1 X 500 μL
100RXN is packaged as 1 X 2.5 mL
800RXN is packaged as 1 X 20 mL

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid precipitable DNA in 30 min. at 74 °C.

Other Notes

View more detailed information on JumpStart REDTaq and ReadyMix products at www.sigma-aldrich.com/hotstart.
For a typical PCR reaction, mix 25 mL of JumpStart REDTaq® ReadyMix with 25 mL of a mixture containing template DNA, primers, and water. Reaction volume can be scaled if desired.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Certificate of Analysis

Certificate of Origin

Stephen J Murphy et al.
Gastroenterology, 145(5), 1098-1109 (2013-08-06)
Increasing grade of pancreatic intraepithelial neoplasia (PanIN) has been associated with progression to pancreatic ductal adenocarcinoma (PDAC). However, the mechanisms that control progression from PanINs to PDAC are not well understood. We investigated the genetic alterations involved in this process. Genomic...
Stephen J Murphy et al.
DNA research : an international journal for rapid publication of reports on genes and genomes, 19(5), 395-406 (2012-09-20)
High-throughput next-generation sequencing provides a revolutionary platform to unravel the precise DNA aberrations concealed within subgroups of tumour cells. However, in many instances, the limited number of cells makes the application of this technology in tumour heterogeneity studies a challenge....
A DNA methylation microarray-based study identifies ERG as a gene commonly methylated in prostate cancer
Schwartzman J, et al.
Epigenetics, 6, 1248-1256 (2011)
Wendy Monger et al.
Archives of virology, 163(6), 1585-1594 (2018-03-02)
A novel virus was discovered in a freeze-dried collection held at SASA, UK, originating from potato (Solanum tuberosum) cv. Nadine. The complete sequence of the viral RNA was determined to be 3674 nucleotides in length encoding five predicted proteins. Based...
Assaad Semaan et al.
Pharmaceutical research, 28(12), 3079-3090 (2011-08-06)
MicroRNA-101 (miR-101) expression is negatively associated with tumor growth and proliferation in several solid epithelial cancers. Enhancer of zeste homolog 2 (EzH2) appears to be a functional target of miR-101. We explore the role of miR-101 and its interaction with...
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