Merck
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P1860

Sigma-Aldrich

Protease Inhibitor Cocktail

for use in tissue culture media, DMSO solution

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Synonym(s):
protease inhibitor
EC Number:
200-664-3
MDL number:
MFCD00677817
NACRES:
NA.77

Quality Level

200

form

DMSO solution

shipped in

dry ice

storage temp.

−20°C

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form

DMSO solution

form

DMSO solution

form

DMSO solution

form

DMSO solution

shipped in

dry ice

shipped in

-

shipped in

-

shipped in

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

300

Quality Level

300

Quality Level

300

General description

The Protease Inhibitor Cocktail is a mixture of protease inhibitors designed to prevent proteolytic degradation of secreted proteins in tissue culture media.

Specificity

Inhibits serine, cysteine, aspartic proteases, and aminopeptidases

Application

This product is specifically optimized for use in tissue culture media and can be added after 48 hours of exposure to fresh medium to ensure the continued inhibition of proteases.

Features and Benefits

Broad specificity: Inhibits serine, cysteine, aspartic proteases, and aminopeptidases

Non-toxic: After 48 hours exposure, the product is non-toxic to adherent cell lines A431, CHO, COS, HepG2 and HeLa; and to Jurkat and HL-60 cell lines grown in suspension.

Contains no metal chelators: Ensures compatibility with downstream applications

Convenient packaging: 1 mL in glass bottle

Easy to use: Use at a dilution of 1:200 or more in tissue culture media to prevent proteolytic degradation of secreted proteins.

Components

Aprotinin
Bestatin
E-64
Leupeptin
Pepstatin A

Other Notes

Mixture of protease inhibitors with broad specificity for the inhibtion of serine, cysteine, aspartic and aminopeptidases. Contains aprotinin, bestatin, leupeptin, E-64, and pepstatin A. Contains no metal chelators

Caution

After 48 hours exposure, the product is non-toxic to adherent cell lines A431, CHO, COS, HepG2 and HeLa; and to Jurkat and HL-60 cell lines grown in suspension.

Quantity

Use at a dilution of 1:200 or more in tissue culture media to prevent proteolytic degradation of secreted proteins.

Physical form

Solution in DMSO (D 2650, Hybri-Max).

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

188.6 °F

Flash Point(C)

87 °C

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