P2317

10X PCR Buffer without MgCl₂

Optimized for routine PCR without MgCl₂

• Synonym(s): Magnesium-free PCR buffer
• NACRES: NA.52

Properties

• Quality Level: 300
• form: liquid
• color: colorless
• application(s): agriculture
• shipped in: wet ice
• storage temp.: −20°C

Description

General description

10X PCR Buffer II was tested at a final concentration of 1X (10mM Tris-HCl, pH 8.3 at 25 °C, 50mM KCl), in reactions containing 1-4mM MgCl₂, each dNTP at 200 μM, primers defining an approximately 500 base pair region of λ DNA at 1.0μM each, λ DNA template at 1ng/100μL, and Taq DNA polymerase at 2.5 units/100μL. The reaction underwent 25 cycles of 94 °C to denature the double stranded DNA, 55 °C to anneal the DNA segments and 72 °C to extend the DNA segments. Following electrophoresis of the reaction products in 1.5% agarose gel, a single band of approximately 500 base pairs was visualized for PCRs containing 1-1.5mM MgCl₂.

Application

10X PCR Buffer without MgCl₂ has been used as a component of polymerase chain reaction (PCR) amplification mixture for the mycotoxigenic mould DNA, parasite DNA and Fusarium oxysporum DNA amplification.It has also been used as a component of the PCR mix for the amplification of nuclear ribosomal internal transcribed spacer (ITS2) and the partial ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit rbcl gene for molecular and morphological characterization of Ulva sp from the Persian Gulf.

10× PCR Buffer without MgCl₂ has been used with Sigma′s PCR enzymes.

Features and Benefits

• This product is tested for the absence of DNase and RNase.
• Suitable for use with magnesium chloride.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable