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General description
Phosphatase Inhibitor Cocktail 1 includes a mixture of inhibitors that will inhibit the L-isozymes of alkaline phosphatase as well as serine/threonine protein phosphatases such as PP1 and PP2A. This product contains microcystinLR, cantharidin, and (−)-p-bromotetramisole. Phosphatase Inhibitor Cocktail 1 has been replaced with P0044.
Application
Phosphatase Inhibitor Cocktail 1 has been used in nuclear protein isolation and electrophoretic mobility shift assay (EMSA) for catenin activation. It has also been used in western blot analysis.
Biochem/physiol Actions
Phosphatases are active regulators of a number of important biological processes. Inhibition of phosphatases by small molecules is useful to study its biological role.
Quantity
Recommended usage is a 100-fold dilution.
Analysis Note
Tested on cell extracts from animal tissues such as bovine liver, human placenta, and rabbit muscle, as well as on extracts from A431 and Jurkat cell lines.
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
188.6 °F
Flash Point(C)
87 °C
Certificate of Analysis
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Certificate of Origin
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Data Sheets
Quotes and Ordering
Articles
Phosphatase Inhibitor Cocktail
The use of phosphatase inhibitors is critical for all types of phosphorylation studies. Compare phosphatase inhibitor cocktails to select the best one for your research.
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