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Phenol solution

Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, BioReagent, for molecular biology

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Tris-equilabrated Phenol
Empirical Formula (Hill Notation):
CAS Number:
Molecular Weight:
EC Number:
MDL number:
PubChem Substance ID:


for molecular biology

Quality Level

product line



≥99.0% (GC)




7.7-8.1 (25 °C)

storage temp.


SMILES string




InChI key


Gene Information

human ... GABRA1(2554)

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General description

A tris-equilibrated phenol solution is a clear liquid capped with a layer of Tris buffer. Phenol solutions can be used for a wide variety of molecular biology applications. Organic compounds like phenols have an aromatic carbocyclic ring with a hydroxyl group attached directly to it. The common name for monohydroxybenzene is phenol.


Phenol solution has been used:

  • as a component of the digestion reaction mix to prepare RNase-free plasmids for in vitro translation in a rabbit reticulocyte lysate
  • for extraction of DNA from Arabidopsis thaliana Rosette leaves
  • in the extraction of DNA from blood on preservation paper by standard phenol-chloroform protocol
  • as a component of extraction buffer to extract RNA from fruit pulp
  • for the extraction of protein from roots
  • for the isolation of fecal DNA
  • for the extraction of caged RNA

Biochem/physiol Actions

The phenol-chloroform extraction method is widely employed to extract nucleic acids. Even though phenol does not completely inhibit RNase activity, its unique characteristic makes it beneficial for the isolation of nucleic acids when used in combination with chloroform and alcohol.


  • 1 bottle Tris-equilabrated Phenol (product number P4557)
  • Equilibration Buffer (product B5658)

Signal Word


Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

Storage Class Code

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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