P4557

Phenol solution

Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, BioReagent, for molecular biology

• Synonym(s): Tris-equilabrated Phenol
• Empirical Formula (Hill Notation): C₆H₆O
• CAS Number: 108-95-2
• Molecular Weight: 94.11
• Beilstein: 6428463
• EC Number: 203-632-7
• MDL number: MFCD00002143
• PubChem Substance ID: 57654529
• NACRES: NA.52

Properties

• grade: for molecular biology
• Quality Level: 200
• product line: BioReagent
• assay: ≥99.0% (GC)
• form: liquid
• pH: 7.7-8.1 (25 °C)
• storage temp.: 2-8°C
• SMILES string: Oc1ccccc1
• InChI: 1S/C6H6O/c7-6-4-2-1-3-5-6/h1-5,7H
• InChI key: ISWSIDIOOBJBQZ-UHFFFAOYSA-N
• Gene Information: human ... GABRA1(2554)

Description

General description

A tris-equilabrated phenol solution is a clear liquid capped with a layer of Tris buffer. Phenol solutions can be used for a wide variety of molecular biology applications, including nucleic acid extractions using standard phenol-chloroform protocols.

Application

Phenol solution has been used:

• as a component of the digestion reaction mix to prepare RNase-free plasmids for in vitro translation in rabbit reticulocyte lysate
• for extraction of protein from roots
• for extraction of DNA from Arabidopsis thaliana Rosette leaves
• in the extraction of DNA from blood on preservation paper by standard phenol-chloroform protocol
• as a component of extraction buffer to extract RNA from fruit pulp

Packaging

100, 100, 400 mL in glass bottle

Components

• 1 bottle Tris-equilabrated Phenol (product number P4557)
• Equilibration Buffer (product B5658)

Safety Information

• Pictograms: GHS05,GHS06,GHS08,GHS09
• Signal Word: Danger
• Hazard Statements: H301 + H311 + H331 - H314 - H341 - H373 - H411
• Precautionary Statements: P202 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
• Storage Class Code: 6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves