PHG0001

HEPES

• Synonym(s): N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid), 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid
• Empirical Formula (Hill Notation): C₈H₁₈N₂O₄S
• CAS Number: 7365-45-9
• Molecular Weight: 238.30
• Beilstein: 883043
• EC Number: 230-907-9
• MDL number: MFCD00006158
• PubChem Substance ID: 329823019
• NACRES: NA.21

Properties

• Quality Level: 400
• biological source: synthetic
• form: powder
• application(s): cell culture | mammalian: suitable
• impurities: Endotoxin, microbial, and trace metals; tested
• pH: 4.7-5.3 (, )
• useful pH range: 6.8 - 8.2
• pKa (25 °C): 7.5
• suitability: suitable for manufacturing use
• foreign activity: Cytotoxicity, DNase, NICKase, RNase, and Protease; tested
• SMILES string: OCCN1CCN(CC1)CCS(O)(=O)=O
• InChI: 1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
• InChI key: JKMHFZQWWAIEOD-UHFFFAOYSA-N

Description

Packaging

100 g in poly bottle

5, 10, 25, 50 kg in poly drum

Application

HEPES is a biological buffer often referred to as a “Good′s” buffer. It is the most commonly used biological buffer since the pKa of HEPES is 7.31 which corresponds to the physiological pH of 7.36. HEPES is considered to be non-toxic to culture cell lines, highly water soluble and provides high solution clarity.

HEPES is used in cell culture media, in biopharma buffer formulations (both upstream and downstream), and in diagnostic reagents. HEPES based buffers are used in purification bioprocesses of antibodies, bioactives, proteins and growth factors.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK Germany: WGK 1
• Flash Point F: Not applicable
• Flash Point C: Not applicable