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Key Documents

PHG0001

SAFC

HEPES

Synonym(s):

4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

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About This Item

Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
Molecular Weight:
238.30
Beilstein:
883043
EC Number:
MDL number:
UNSPSC Code:
12161700
NACRES:
NA.21
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biological source

synthetic

Quality Level

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, microbial, and trace metals; tested

pH

4.7-5.3 (, )

useful pH range

6.8-8.2

pKa (25 °C)

7.5

suitability

suitable for manufacturing use

foreign activity

Cytotoxicity, DNase, NICKase, RNase, and Protease; tested

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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H4034PHR1428H6147
HEPES

PHG0001

HEPES

HEPES BioPerformance Certified, ≥99.5% (titration), suitable for cell culture

H4034

HEPES

HEPES Pharmaceutical Secondary Standard; Certified Reference Material

PHR1428

HEPES

HEPES BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)

H6147

HEPES

biological source

synthetic

biological source

-

biological source

-

biological source

-

form

powder

form

crystalline powder

form

-

form

powder

impurities

Endotoxin, microbial, and trace metals; tested

impurities

endotoxin and total aerobic microbial count, tested

impurities

-

impurities

-

suitability

suitable for manufacturing use

suitability

suitable for Western blot, suitable for component for culture media

suitability

-

suitability

-

useful pH range

6.8-8.2

useful pH range

6.8-8.2

useful pH range

6.8-8.2

useful pH range

6.8-8.2

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable, competitive inhibition ELISA: suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

technique(s)

cell culture | embryo: suitable

General description

Our SAFC® portfolio of high-quality raw materials for use in biopharmaceutical processing withstands strict quality control procedures plus the documentation and expertise to help our customers meet requirements as defined by the M-Clarity Program.

M-Clarity Program

Buffer quality is vital for the success of biopharmaceutical processes, because buffers are indispensable in nearly every production step.

Our broad portfolio of buffer materials manufactured under appropriate controls is tailored to your needs. Ranging from non-GMP grades for low-risk application, to IPEC-PQG GMP for higher-risk applications, we have products covering all your manufacturing needs.

Application

HEPES is a biological buffer often referred to as a “Good′s” buffer. It is the most commonly used biological buffer since the pKa of HEPES is 7.31 which corresponds to the physiological pH of 7.36. HEPES is considered to be non-toxic to culture cell lines, highly water soluble and provides high solution clarity.

HEPES is used in cell culture media, in biopharma buffer formulations (both upstream and downstream), and in diagnostic reagents. HEPES based buffers are used in purification bioprocesses of antibodies, bioactives, proteins and growth factors.

Legal Information

SAFC is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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