Merck
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PIC0004

Sigma-Aldrich

ReadyShield® Protease Inhibitor Cocktail

for use in purification of Histidine-tagged proteins, Non-freezing solution

Synonym(s):
Protease inhibitor cocktail

description

Purification of Histidine-Tagged Proteins

form

ready-to-use solution

quality

200

packaging

pkg of 1 mL (in amber polypropylene vial)
pkg of 5 mL (in amber polypropylene vial)

storage temp.

−20°C

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This Item
PIC0002PIC0006PIC0001
form

ready-to-use solution

form

ready-to-use solution

form

ready-to-use solution

form

(non-freezing liquid formulation )

packaging

pkg of 1 mL (in amber polypropylene vial), pkg of 5 mL (in amber polypropylene vial)

packaging

pkg of 1 mL (in amber polypropylene vial), pkg of 5 mL (in amber polypropylene vial)

packaging

pkg of 1 mL (in amber polypropylene vial), pkg of 5 mL (in amber polypropylene vial)

packaging

pkg of 1 mL (in amber polypropylene vial), pkg of 5 mL (in amber polypropylene vial)

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

description

Purification of Histidine-Tagged Proteins

description

Mammalian Cell and Tissue Extracts

description

Tissue Culture Media

description

Fungal and Yeast Extracts

quality

200

quality

200

quality

200

quality

200

Extracts, Lysate, Cell Extracts, Tissue Extracts, Mammalian Tissue Extract, Bacterial lysate, Recombinant extracts, fusion proteins, Electrophoresis, peptidase inhibitor, proteinase inhibitor

Extracts, Lysate, Cell Extracts, Tissue Extracts, Mammalian Tissue Extract, Electrophoresis, peptidase inhibitor, proteinase inhibitor, Cell culture

Extracts, Lysate, Cell Extracts, Tissue Extracts, Mammalian Tissue Extract, Bacterial lysate, Electrophoresis, peptidase inhibitor, proteinase inhibitor, Cell culture

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General description

This particular protease inhibitor cocktail omits chelators (e.g., EDTA), to optimize its use for purification of histidine-tagged proteins.1 It contains inhibitors with a broad specificity for serine, cysteine, and acid proteases, and aminopeptidases. The cocktail has been tested on extracts from E. coli and insect cells This cocktail is supplied as a ready-to-use solution using a proprietary non-freezing formulation. ReadyShield PIC0004 is a non-freezing formulation that contains the same inhibitors as is the DMSO based cocktail P8849.

Features and Benefits

  • Does not freeze in storage for quick, convenient use.
  • Tailored for use with Histidine fusion tagged proteins.
  • Suggested as 100 X solution.
  • Reclosable screw-top vials.
  • Can be stored in cooler for approx. 1 month or room temperature for a week.

Preparation Note

The recommended dilution of the cocktail in the biological extract is 1 ml of the cocktail per 100 ml of lysate from 20 g (wet weight) of E. coli cells or 10 g (wet weight) of baculovirus-infected insect (Spodoptera frugiperda) cells.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

ReadyShield is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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