PIC0006

ReadyShield^® Protease Inhibitor Cocktail

for use in tissue culture media, Non-freezing solution

• Synonym(s): Protease inhibitor cocktail

Properties

• description: Tissue Culture Media
• form: ready-to-use solution
• quality: 200
• packaging: pkg of 1 mL (in amber polypropylene vial); pkg of 5 mL (in amber polypropylene vial)
• storage temp.: −20°C

Description

General description

This product is a mixture of protease inhibitors with a broad specificity for the inhibition of serine, cysteine, aspartic proteases and aminopeptidases. It is designed to be used as a supplement to tissue culture medium for the prevention of secreted proteins degradation. The product has been found to be non-toxic after a 48 hours exposure to the following adherent cell lines: A431, CHO, COS, HepG2, and HeLa, and to following non-adherent cell lines: Jurkat and HL-60. This cocktail is supplied as a ready-to-use solution using a proprietary non-freezing formulation. ReadyShield PIC0006 is a non-freezing formulation that contains the same inhibitors as is the DMSO based cocktail P1860.

Features and Benefits

• Does not freeze in storage for quick, convenient use.
• Inhibits serine, cysteine, aspartic proteases and aminopeptidases.
• Tailored for use in tissue culture media.
• Suggested as 100 X solution.
• Reclosable screw-top vials.
• Can be stored in cooler for approx. 1 month or room temperature for a week.

Preparation Note

It is recommended to determine the appropriate dilution for each specific cell line. This testing should begin with a dilution of at least a 200-fold to prevent deleterious effect of the solvent to cell growth.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

ReadyShield is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable