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PLN350

GenElute™ Plasmid Miniprep Kit

greener alternative

sufficient for 350 purifications

Synonym(s):

GenElute™ Plasmid Kit, plasmid extraction kit, plasmid isolation kit, plasmid purification kit, Gen Elute

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About This Item

NACRES:
NA.52
UNSPSC Code:
41105501
Pricing and availability is not currently available.
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usage

sufficient for 350 purifications

Quality Level

200

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

technique(s)

DNA purification: suitable

test parameters

: <30 min kit run time, sample volume: 1-5 mL bacterial culture

greener alternative category

Aligned,

storage temp.

15-25°C

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This Item
NA0160NA0150PLN70
GenElute&#8482; Plasmid Miniprep Kit sufficient for 350&#160;purifications

Sigma-Aldrich

PLN350

GenElute™ Plasmid Miniprep Kit

GenElute&#8482; HP Plasmid Miniprep Kit sufficient for 350&#160;preparations

Sigma-Aldrich

NA0160

GenElute™ HP Plasmid Miniprep Kit

GenElute&#8482; HP Plasmid Miniprep Kit sufficient for 70&#160;preparations

Sigma-Aldrich

NA0150

GenElute™ HP Plasmid Miniprep Kit

GenElute&#8482; Plasmid Miniprep Kit sufficient for 70&#160;purifications

Sigma-Aldrich

PLN70

GenElute™ Plasmid Miniprep Kit

usage

sufficient for 350 purifications

usage

sufficient for 350 preparations

usage

sufficient for 70 preparations

usage

sufficient for 70 purifications

technique(s)

DNA purification: suitable

technique(s)

DNA extraction: suitable

technique(s)

DNA extraction: suitable

technique(s)

DNA purification: suitable

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

test parameters

: <30 min kit run time, sample volume: 1-5 mL bacterial culture

test parameters

: <30 min kit run time, sample volume: 1-5 mL bacterial culture

test parameters

-

test parameters

: <30 min kit run time, sample volume: 1-5 mL

General description

Click here to view the kit protocol
The GenElute Plasmid Miniprep Kit offers a simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures. By combining silica-binding technology and the convenience of a spin column format, up to 15 μg of high copy plasmid DNA can be recovered from 1-5 mL of E. coli culture in less than 30 minutes. Note that actual yield and optimal volume of culture to use depend on the plasmid and the culture medium.

An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto silica in the presence of high salts. Contaminants are then removed by a spin-wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

Application

The DNA is ready for immediate use in applications such as restriction enzyme digestion, cloning, PCR, transformation, transcription, conventional, and automated sequencing.
The kit has been used for the purification of plasmid DNA of Dirofilaria repens.

Features and Benefits

  • Purified plasmid DNA in less than 30 minutes
  • Purify up to 15 μg of plasmid DNA
  • No detectable genomic DNA or RNA contamination
  • No phenol/chloroform extraction or alcohol precipitation required

Other Notes

For additional information, please see www.sigma-aldrich.com/genelutehp.

Legal Information

GenElute is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS
  • C2112Column Preparation SolutionSDS
  • R6148RNase A solutionSDS
  • L1912Lysis SolutionSDS
  • R1149Resuspension Solution, Add RNase A before use. After addition of RNAse, store at 2-8ºC. )SDS

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Central nervous system

Storage Class

3 - Flammable liquids

wgk

WGK 2

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000524138
0000524138
0000451820
0000451820
0000434221

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Dirofilaria repens: emergence of autochthonous human infections in the Czech Republic (case reports).
Mateju J
BMC Infectious Diseases, 16, 171-171 (2016)
Simeon S Andrews et al.
The Journal of biological chemistry, 294(30), 11549-11558 (2019-06-12)
Two-hybrid systems can be used for investigating protein-protein interactions and may provide important information about gene products with unknown function. Despite their success in mapping protein interactions, two-hybrid systems have remained mostly untouched by improvements in next-generation DNA sequencing. The
Vanessa Meier-Stephenson et al.
The Journal of biological chemistry, 296, 100589-100589 (2021-03-29)
Approximately 250 million people worldwide are chronically infected with the hepatitis B virus (HBV) and are at increased risk of developing cirrhosis and hepatocellular carcinoma. The HBV genome persists as covalently closed circular DNA (cccDNA), which serves as the template
Jing Yang et al.
Preparative biochemistry & biotechnology, 50(6), 529-537 (2020-01-10)
As a lincosamide antibiotic, lincomycin is still important for treating diseases caused by Gram-positive bacteria. Manufacturing of lincomycin needs efforts to, e.g. maximize desirable species and minimizing unwanted fermentation byproducts. Analysis of the lincomycin biosynthetic gene cluster of Streptomyces lincolnensis
Richard A Urbanowicz et al.
Cell, 167(4), 1079-1087 (2016-11-05)
The 2013-2016 outbreak of Ebola virus (EBOV) in West Africa was the largest recorded. It began following the cross-species transmission of EBOV from an animal reservoir, most likely bats, into humans, with phylogenetic analysis revealing the co-circulation of several viral
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Product Information Sheet
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Global Trade Item Number

SKUGTIN
PLN350-1KT04061836681326

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