R1383

RPMI-1640 Medium

With L-glutamine, without glucose and sodium bicarbonate, powder, suitable for cell culture

• Synonym(s): Roswell Park Memorial Institute 1640 medium
• MDL number: MFCD00217820
• NACRES: NA.75

Properties

• form: powder
• technique(s): cell culture | mammalian: suitable
• components: L-glutamine: yes; phenol red: yes; sodium pyruvate: no; HEPES: no; NaHCO₃: no
• shipped in: ambient
• storage temp.: 2-8°C

Description

General description

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage-dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells. This medium is suitable for culturing human normal and neoplastic leukocytes.

Application

RPMI-1640 Medium has been used as a cell culture medium to culture human MCF-7 breast cancer cells and human castrate-resistant prostate cancer (CRPC) cell lines for various cell culture experiments. It has also been used to culture human islets to assess the oxidative stress response in primary human islets.

Quantity

Formulated to contain 8.4 grams of powder per liter of medium.

Reconstitution

Supplement with 2.0 g/L sodium bicarbonate.

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable