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Human Tumor Necrosis Factor α ELISA Kit

for serum, plasma, cell culture supernatants and urine

Anti-NR2B, TNF-alpha

species reactivity



kit of 96 wells (12 strips x 8 wells)


ELISA: suitable
capture ELISA: suitable


sample type cell culture supernatant(s)
sample type plasma
sample type urine
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 30 pg/mL
standard curve range: 24.58-6000 pg/mL

detection method


shipped in

wet ice

storage temp.


Gene Information

human ... TNF(7124)

General description

The Human TNF-α ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TNF-α in serum, plasma, cell culture supernatants and urine.


Recombinant Human TNF-α


For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human Tumor Necrosis Factor α ELISA Kit has been used to measure the concentration of TNF-α in pre-adipocyte growth medium, blood sample and in human bronchial epithelial cell (BEAS 2B) culture supernatantand normal human keratinocytes (NHK) cell culture supernatant.

Biochem/physiol Actions

Tumor necrosis factor- α (TNF-α) helps in regulating immune response and it acts as a proinflammatory cytokine. TNF-α is implicated in the immune pathogenesis of various autoimmune, inflammatory diseases and hepatitis B virus (HBV) infection. Overexpression of the protein has been observed in serum from oral lichen planus (OLP) patients. Mutation in the gene promotor increases the risk of susceptibility to diffuse large B-cell lymphoma (DLBCL) and marginal zone lymphoma.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)

  • RABSTOP3ELISA Stop Solution (Item I)

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  • RABWASH420X Wash Buffer (Item B)



Signal Word


Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials



Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Human leukocyte antigen class I and II alleles in non-Hodgkin lymphoma etiology
Wang SS, et al.
Blood, 115(23), 4820-4823 (2010)
Luca Fusaro et al.
Frontiers in bioengineering and biotechnology, 8, 281-281 (2020-04-23)
Cardiovascular diseases represent the leading cause of death in developed countries. Modern surgical methods show poor efficiency in the substitution of small-diameter arteries (<6 mm). Due to the difference in mechanical properties between the native artery and the substitute, the
Tanshinone II is a potent candidate for treatment of lipopolysaccharide-induced acute lung injury in rat model
Li L, et al.
Oncology Letters, 15(2), 2550-2554 (2018)
In vitro evaluation of the anti-inflammatory activity of an oral administration product containing collagen peptides, Delphinol ? , vitamin c and hibiscus
Nunes S, et al.
Surgical And Cosmetic Dermatology (2018)
Association between TNF alpha-308 G/A polymorphism and oral lichen planus (OLP): a meta-analysis
Zhou Yand Vieira AR
Journal of Applied Oral Science : Revista FOB, 26 (2018)

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