Merck
All Photos(2)

RAB0480

Sigma-Aldrich

Rat Tumor Necrosis Factor α ELISA Kit

for cell and tissue lysates

Synonym(s):
Anti-IFF, Anti-IFB, TNF-alpha, Anti-IFN-beta
NACRES:
NA.32

species reactivity

rat

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable
capture ELISA: suitable

input

sample type tissue lysate
sample type cell lysate

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 25 pg/mL
standard curve range: 82.3-20000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

rat ... Tnf(24835)

General description

The Rat TNF-α ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat TNF-α cell lysate and tissue lysate.

Immunogen

Recombinant Rat TNF-α

Application

For research use only. Not for use in diagnostic procedures.
Rat Tumor Necrosis Factor α ELISA Kit has been used to measure rat tumor necrosis-α (TNF-α) in cell lysate and tissue lysate.

Biochem/physiol Actions

Tumor necrosis factor-α (TNF-α) affects various cellular processes such as blood-brain barrier, inflammatory, thrombogenic, and vascular changes following brain damage. TNF-α expression is upregulated in various central nervous system disorders, including Alzheimer′s disease, multiple sclerosis, Parkinson′s disease, meningococcal meningitis and HIV infection. Elevated expression of TNF-α in brain and blood in response to lipopolysaccharide might increase brain stem thrombosis and hemorrhage and stroke sensitivity/risk in hypertensive rats. Tumor necrosis factor-α activates polymorphonuclear leukocyte NADPH oxidase, thereby facilitates development of systemic oxidative stres (OS), inflammation and hypertension in rats.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.
Description
SDS

  • RABDIL6ELISA 5X Sample Diluent Buffer (Item D2)

  • RABELADEELISA 5X Assay/Sample Diluent Buffer E (Item E2)

  • RABLYSIS12X Cell Lysis Buffer (Item J)

  • RABSTOP3ELISA Stop Solution (Item I)

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  • RABWASH420X Wash Buffer (Item B)

Pictograms

Corrosion

Signal Word

Warning

Hazard Statements

Precautionary Statements

Hazard Classifications

Met. Corr. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Product Information Sheet

Quotes and Ordering

Diosmin Modulates the NF-kB Signal Transduction Pathways and Downregulation of Various Oxidative Stress Markers in Alloxan-Induced Diabetic Nephropathy.
Ahmed S
Inflammation, 39(5), 1783-1797 (2016)
R A Ajala-Lawal et al.
Archives of physiology and biochemistry, 126(2), 107-115 (2018-10-06)
This study investigated the influence of betulinic acid on high-fructose diet-induced metabolic syndrome in rats. Oral administration of betulinic acid significantly reversed high-fructose diet-mediated increase in body mass index and blood glucose. Furthermore, betulinic acid restored high-fructose diet-mediated alterations in
Meijuan Chen et al.
Gastroenterology research and practice, 2019, 8723460-8723460 (2019-10-01)
Phosphatidylcholine (PC) is the major surface-active phospholipid and creates a hydrophobic nature to the surface. It has been reported to reverse the progression of liver fibrosis and to improve liver function. The aim of the present study was to evaluate
Vu Thi Thu et al.
The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology, 20(3), 305-314 (2016-05-11)
Inflammatory and fibrotic responses are accelerated during the reperfusion period, and excessive fibrosis and inflammation contribute to cardiac malfunction. NecroX compounds have been shown to protect the liver and heart from ischemia-reperfusion injury. The aim of this study was to
Nanik Sundari et al.
Journal of toxicology, 2018, 2642714-2642714 (2019-01-12)
Phaleria macrocarpa is one of the Indonesian herbal plants which has been shown to have a hepatoprotective effect. This study was conducted to evaluate the protective effect of water extract of mahkota dewa (Phaleria macrocarpa) in liver fibrosis and to

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service