All Photos(4)




≥99% (GC)

N-(3-Aminopropyl)-1,4-diaminobutane, 1,8-Diamino-4-azaoctane
Linear Formula:
CAS Number:
Molecular Weight:
EC Number:
MDL number:
PubChem Substance ID:

Quality Level

biological source



≥99% (GC)


solid or liquid (clear)


DNase, none detected
RNase, none detected
protease, none detected

refractive index

n20/D 1.479 (lit.)


22-25 °C


water: 50 mg/mL, clear to very slightly hazy, colorless to very faintly yellow


0.925 g/mL at 25 °C (lit.)

storage temp.

room temp

SMILES string




InChI key


Gene Information

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General description

The enzyme nitric oxide synthase (nNOS) is involved in the production of nitric oxide.


Spermidine has been used:
  • in the preparation of 5x transcription buffer
  • in the treatment of double-stranded RNA along with other reagents to increase the uptake of dsRNA by nematodes
  • as a standard in the analytical study of rat brain polyamines (185)


1, 5, 25 g in glass bottle

Biochem/physiol Actions

Inhibits neuronal nitric oxide synthase (nNOS). Binds and precipitates DNA; may be used for purification of DNA binding proteins. Stimulates T4 polynucleotide kinase activity.


Solutions should be sterile-filtered, not autoclaved, if sterile solution is necessary. Spermidine deaminates with time; solutions should be stored frozen. Prepare new solutions frequently.

Other Notes

Store under inert gas. Air sensitive; strongly hygroscopic.



Signal Word


Hazard Statements

Hazard Classifications

Eye Dam. 1 - Skin Corr. 1B

Storage Class Code

8A - Combustible, corrosive hazardous materials



Flash Point(F)

233.6 °F - closed cup

Flash Point(C)

112 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

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    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Is this a spermidine solution or a neat liquid, and what is the concentration?

    Based on a density of 0.925 g/mL and a molecular weight of 145.25, this neat liquid has an effective molarity of 6.4 M.

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In vitro Reconstitution Assay of miRNA Biogenesis by Arabidopsis DCL1.
Wang T
Bio-protocol, 5(8), e1454-e1454 (2015)
Quantitative Detection of Double-Stranded RNA-Mediated Gene Silencing of Parasitism Genes in Heterodera glycines.
Sukno SA
Journal of Nematology, 39(2), 145-152 (2007)
Evidence for the contribution of NOS1 gene polymorphism (rs3782206) to prefrontal function in schizophrenia patients and healthy controls.
Zhang Z
Neuropsychopharmacology, 40(6), 1383-1394 (2015)
Michi Miura et al.
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology, 28(3-4), 381-393 (2020-10-19)
CUT&RUN is a powerful tool to study protein-DNA interactions in vivo. DNA fragments cleaved by the targeted micrococcal nuclease identify the footprints of DNA-binding proteins on the chromatin. We performed CUT&RUN on human lung carcinoma cell line A549 maintained in
Eugenia Morselli et al.
Aging, 1(12), 961-970 (2010-02-17)
Although autophagy has widely been conceived as a self-destructive mechanism that causes cell death, accumulating evidence suggests that autophagy usually mediates cytoprotection, thereby avoiding the apoptotic or necrotic demise of stressed cells. Recent evidence produced by our groups demonstrates that

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