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S5150

Sodium chloride solution

5 M in H2O, BioReagent, Molecular Biology, suitable for cell culture

Synonym(s):

Halite, NaCl, sodium chloride

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About This Item

Linear Formula:
NaCl
CAS Number:
Molecular Weight:
58.44
UNSPSC Code:
12352302
NACRES:
NA.25
PubChem Substance ID:
EC Number:
231-598-3
Beilstein/REAXYS Number:
3534976
MDL number:
Grade:
Molecular Biology
Form:
liquid
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grade

Molecular Biology

Quality Level

sterility

0.2 μm filtered

product line

BioReagent

form

liquid

concentration

5 M in H2O

technique(s)

ChIP: suitable, DNA extraction: suitable, cell culture | mammalian: suitable

impurities

DNAse, none detected, Endonuclease, none detected, Exonuclease, none detected, NICKase, none detected, Protease, none detected, RNAse, none detected, <1.0 EU/mL Endotoxin level, <16 cfu/mL Bioburden

pH

6.0-8.0

density

1.190 g/cm3

anion traces

phosphate (PO43-): ≤5 ppm

cation traces

K: ≤50 ppm, heavy metals (as Pb): ≤5 ppm

suitability

suitable for Western blot, suitable for molecular biology

application(s)

agriculture

foreign activity

DNAse, Exonuclease; RNAse; NICKase, Endonuclease; Protease, none detected

SMILES string

[Na+].[Cl-]

InChI

1S/ClH.Na/h1H;/q;+1/p-1

InChI key

FAPWRFPIFSIZLT-UHFFFAOYSA-M

Application

Sodium chloride solution has been used as a component of mycobacterial lysis buffer to extract DNA.[1] It has also been used along with Dulbecco′s phosphate-buffered saline (DPBS) in the preparation of degassed buffer to elute plasma samples.

Preparation Note

Prepared in 18 megohm water.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

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form

liquid

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liquid

Quality Level

200

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100

Quality Level

200

Quality Level

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grade

Molecular Biology

grade

Molecular Biology

grade

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grade

-

cation traces

K: ≤50 ppm, heavy metals (as Pb): ≤5 ppm

cation traces

Al: ≤1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤20 mg/kg, Li: ≤1 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg

cation traces

-

cation traces

-

anion traces

phosphate (PO43-): ≤5 ppm

anion traces

-

anion traces

-

anion traces

-

pH

6.0-8.0

pH

5.0-7.0 (25 °C)

pH

-

pH

6.5-7.5


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Storage Class

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)



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Protocols

Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit


Gender-dependent effect of Gpbar1 genetic deletion on the metabolic profiles of diet-induced obese mice
Vassileva G, et al.
The Journal of Endocrinology, 205(3), 225-232 (2010)
Tim J Bull et al.
Journal of clinical microbiology, 41(7), 2915-2923 (2003-07-05)
Mycobacterium avium subsp. paratuberculosis is a robust and phenotypically versatile pathogen which causes chronic inflammation of the intestine in many species, including primates. M. avium subsp. paratuberculosis infection is widespread in domestic livestock and is present in retail pasteurized cows'
Francis Kim et al.
JAMA, 311(1), 45-52 (2013-11-19)
Hospital cooling improves outcome after cardiac arrest, but prehospital cooling immediately after return of spontaneous circulation may result in better outcomes. To determine whether prehospital cooling improves outcomes after resuscitation from cardiac arrest in patients with ventricular fibrillation (VF) and



Global Trade Item Number

SKUGTIN
S5150-1L04061836944605
S5150-4L04061836944742
S5150-1.5ML04061838080400
S5150-8ML04061838238283

Questions

1–2 of 2 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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