All Photos(3)

SAB5500001

Sigma-Aldrich

Anti-beta-Actin antibody, Rabbit monoclonal

clone SP124, recombinant, expressed in proprietary host, affinity isolated antibody

biological source

rabbit

Quality Level

recombinant

expressed in proprietary host

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

SP124, monoclonal

species reactivity

human (tested)

species reactivity (predicted by homology)

mouse, chicken, pig, rat, hamster, dog, frog, bovine, rabbit

technique(s)

flow cytometry: 1:100
immunoblotting: 1:200
immunohistochemistry: 1:200

isotype

IgG

UniProt accession no.

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... ACTB(60)

General description

ACTB (actin β) gene codes for β-actin. It is a cytoskeletal housekeeping protein. It is present in the cytoplasm. This gene is located on human chromosome 7p22.1.
Beta-actin is a nonmuscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. Antibody to beta-actin provides a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.

Immunogen

Synthetic peptide near the N-terminus of human beta-Actin protein.

Application

Anti-Actin-Beta antibody has been used in western blotting.

Biochem/physiol Actions

β-Actin modulates cell growth, migration and the G-actin pool. Mutations in ACTB leads to pleiotropic developmental disorder.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

0.1 ml rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Lin Liao et al.
Experimental cell research, 359(1), 266-274 (2017-07-22)
We have established that annexin A2 (ANXA2) is an important factor in the experimental hepatopulmonary syndrome (HPS) serum-induced proliferation of pulmonary arterial smooth muscle cells (PASMCs). However, the detailed mechanism remains unclear. ANXA2 translocated to the caveolin-enriched microdomains (caveolae) in
Soy isoflavones protect against H2O2-induced injury in human umbilical vein endothelial cells
Jin L, et al.
Molecular Medicine Reports, 12, 4476-4482 (2015)
Exosomes Derived from Squamous Head and Neck Cancer Promote Cell Survival after Ionizing Radiation
Mutschelknaus L, et al.
PLoS ONE (2016)
Nicotinic ?7 receptor inhibits the acylation stimulating protein?induced production of monocyte chemoattractant protein?1 and keratinocyte?derived chemokine in adipocytes by modulating the p38 kinase and nuclear factor??B signaling pathways
Jiao ZY, et al.
Molecular Medicine Reports, 14(4), 2959-2966 (2016)
Xian-Lu Song et al.
Journal of Cancer, 8(13), 2501-2510 (2017-09-14)
miRNAs play critical role in the development and progression of prostate cancer. Here we studied the role of miR-618 in prostate cancer migration and invasion. miR-618 was downregulated in metastatic androgen-independent prostate cancer (AIPC), patients with low miR-618 had poor

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