Merck
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SRP3045

Sigma-Aldrich

Follistatin human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):
FS, activins-binding protein
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

0.1-0.4 μg/mL

mol wt

31.5 kDa

packaging

pkg of 20 μg

technique(s)

cell culture | mammalian: suitable

impurities

<1.0 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... FST(10468)

General description

Follistatin is a secreted protein that binds to ligands of the TGF (transforming growth factor)-β family and regulates their activity by inhibiting their access to signaling receptors. Follistatin is expressed in the pituitary, ovaries, decidual cells of the endometrium, and in some other tissues. The gene is mapped to human chromosome 5q11.2. Recombinant human Follistatin is a 31.5kDa protein containing 288 amino acids. Its primary structure contains three cysteine-rich domains (called FS domains), each followed by a protease-inhibitory kazal domain.

Application

Follistatin human has been used to suppress myostatin signaling. It has also been used as an inhibitor of activin A.

Biochem/physiol Actions

Follistatin is a multifunctional protein. It was originally discovered as activin antagonists whose activity suppresses expression and secretion of the pituitary hormone FSH (follicle stimulating hormone). In addition to being a natural antagonist, follistatin can inhibit the activity of other TGF (transforming growth factor)-β ligands including BMP (bone morphogenetic protein)-2,-4,-6,-7, Myostatin, GDF-11 (growth differentiation factor 11), and TGF-b1. In gonads, it is responsible for oocyte competence and embryo development. In the pituitary gland, it causes cyclic changes in FSH production in the estrous cycle. Follistatin also participates in bone metabolism. It controls inflammation, fibrosis and tissue repair. Levels of plasma follistatin increases in polycystic ovary syndrome and type 2 diabetes. Follistatin might also be involved in tumor progression, including apoptosis, angiogenesis, and metastasis.

Sequence

GNCWLRQAKN GRCQVLYKTE LSKEECCSTG RLSTSWTEED VNDNTLFKWM IFNGGAPNCI PCKETCENVD CGPGKKCRMN KKNKPRCVCA PDCSNITWKG PVCGLDGKTY RNECALLKAR CKEQPELEVQ YQGRCKKTCR DVFCPGSSTC VVDQTNNAYC VTCNRICPEP ASSEQYLCGN DGVTYSSACH LRKATCLLGR SIGLAYEGKC IKAKSCEDIQ CTGGKKCLWD FKVGRGRCSL CDELCPDSKS DEPVCASDNA TYASECAMKE AACSSGVLLE VKHSGSCN

Physical form

Lyophilized from 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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