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SRP3071

Sigma-Aldrich

IL-10 human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(s):
B-TCGF, TGIF, CSIF
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

assay

≥98% (HPLC)
≥98% (SDS-PAGE)

form

lyophilized

potency

≤2.0 ng/mL ED50

mol wt

18.6 kDa

packaging

pkg of 10 μg

application(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... IL10(3586)

General description

IL-10 (interleukin 10) is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. The gene is mapped to human chromosome 1q31-1q32. Recombinant human IL-10 is an 18.6kDa protein of 161 amino acid residues. Human IL-10 active on murine cells, but murine IL-10 is inactive on human cells.

Application

IL-10 human has been used to study the effect of IL-10 on endothelial nitric oxide synthase and nitric oxide in African American human umbilical vein endothelial cells. It has also been used to study the effect of IL-10 on leukotriene (LT) A4 hydrolase and LTC4 synthase protein expression in and secretion from, an inflamed porcine endometrium.

Biochem/physiol Actions

IL-10 (interleukin 10) is an anti-inflammatory cytokine. IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and TNF (tumor necrosis factor)-α. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions. Polymorphisms in the IL-10 gene are associated with higher risk of preeclampsia, ankylosing spondylitis, nasopharyngeal carcinoma and basal cell carcinoma. Polymorphism in IL-10 at -1082A/G is associated with diabetic nephropathy.

Sequence

MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N

Physical form

Lyophilized from 5 mM Sodium Phosphate, pH 7.2.

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in 5 mM Sodium Phosphate, pH 7.2 to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Storage Class Code

13 - Non Combustible Solids

WGK Germany

WGK 3

Flash Point F

Not applicable

Flash Point C

Not applicable

Certificate of Analysis

Certificate of Origin

Effect of Interleukin-10 and Laminar Shear Stress on Endothelial Nitric Oxide Synthase and Nitric Oxide in African American Human Umbilical Vein Endothelial Cells.
Babbitt DM, et al.
Ethnicity & Disease, 25, 413-418 (2015)
TNF-a and IL10 polymorphisms interaction increases the risk of ankylosing spondylitis in Chinese Han population.
Wang NG, et al.
International Journal of Clinical and Experimental Pathology, 8, 15204-15209 (2015)
Interleukin-10 polymorphisms and nasopharyngeal carcinoma risk: a meta-analysis.
Yu YF, et al.
Genetics and molecular research : GMR, 14, 18945-18957 (2015)
Investigation on the association between inerleukin-10 -592C/A, 819C/T and -1082A/G gene polymorphisms and development of diabetic nephrophathy.
Yin Q, et al.
International Journal of Clinical and Experimental Pathology, 8, 15216-15221 (2015)
Clinical significance of IL-2 and IL-10 gene polymorphisms and serum levels in patients with basal-cell carcinoma.
Sobjanek M, et al.
Biomarkers in Medicine, 10, 185-195 (2016)

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