T0699
liquid
6.1 N
~100 % (w/v)
OC(=O)C(Cl)(Cl)Cl
1S/C2HCl3O2/c3-2(4,5)1(6)7/h(H,6,7)
YNJBWRMUSHSURL-UHFFFAOYSA-N
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Danger
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1A - STOT SE 3
Respiratory system
8B - Non-combustible, corrosive hazardous materials
WGK 2
Not applicable
Not applicable
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Proteinase K (EC 3.4.21.64) activity can be measured spectrophotometrically using hemoglobin as the substrate. Proteinase K hydrolyzes hemoglobin denatured with urea, and liberates Folin-postive amino acids and peptides. One unit will hydrolyze hemoglobin to produce color equivalent to 1.0 μmol of tyrosine per minute at pH 7.5 at 37 °C (color by Folin & Ciocalteu's Phenol Reagent).
Objective: To standardize a procedure for the enzymatic determination of Glucose-6-phosphatase activity
This procedure may be used for determination of Pepsin activity using hemoglobin as the substrate. It is a spectrophotometric stop rate determination.
This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate.
To standardize a procedure for the determination of the enzymatic assay of choloylglycine hydrolase.
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