T5525

3,3′,5,5′-Tetramethylbenzidine

tablet, 1 mg substrate per tablet

• Synonym(s): TMB substrate, TMB, Sure Blue TMB, BM blue, TMB Blotting Plus
• Linear Formula: [-C₆H₂(CH₃)₂-4-NH₂]₂
• CAS Number: 54827-17-7
• Molecular Weight: 240.34
• Beilstein: 2808541
• MDL number: MFCD00007748
• PubChem Substance ID: 24900293
• NACRES: NA.83

Properties

• Quality Level: 200
• form: tablet
• mp: 168-171 °C (lit.)
• storage temp.: 2-8°C
• SMILES string: Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2
• InChI: 1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3
• InChI key: UAIUNKRWKOVEES-UHFFFAOYSA-N

Description

Application

3,3′,5,5′-Tetramethylbenzidine has been used as a substrate in competitive enzyme-linked immunosorbent assay (ELISA) and indirect ELISA.

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H₂SO₄ (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves