T7539

Tumor Necrosis Factor-α from mouse

TNF-α, recombinant, expressed in E. coli, powder, suitable for cell culture

• Synonym(s): mTNF-α, TNF-α
• MDL number: MFCD00164823
• NACRES: NA.77

Properties

• Quality Level: 200
• biological source: mouse
• recombinant: expressed in E. coli
• assay: ≥95%
• form: powder
• potency: 0.01-0.5 ng/mL EC₅₀
• mol wt: 17 kDa
• packaging: pkg of 10 μg; pkg of 50 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): cell culture | mammalian: suitable
• impurities: ≤1 EU/μg protein endotoxin
• color: white
• solubility: water: soluble
• UniProt accession no.: P06804
• storage temp.: −20°C
• Gene Information: mouse ... Tnf(21926)

Description

Application

Tumor Necrosis Factor-α from mouse, a potent proinflammatory cytokine has been used:

• to activate MS1 endothelium cells
• to induce lipolysis in adipocytes
• to study its effect on liganded and unliganded glucocorticoid receptor (GR) in interleukin (IL)-6 and IL-8 gene regulation

Biochem/physiol Actions

Tumor necrosis factor-α (TNF-α), also known as cachectin, is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-α converting enzyme (TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. TNF-α plays roles in antitumor activity, immune modulation, inflammation, anorexia, cachexia, septic shock, viral replication and hematopoiesis. TNF-α is expressed by a great variety of cells, with numerous inductive and suppressive agents. Primarily, TNF-α is produced by macrophages in response to immunological challenges such as bacteria (lipopolysaccharides), viruses, parasites, mitogens and other cytokines. TNF-α is cytotoxic for many transformed cells (its namesake activity) but in normal diploid cells, it can stimulate proliferation (fibroblasts), differentiation (myeloid cells) or activation (neutrophils). TNF-α also shows antiviral effects against both DNA and RNA viruses and it induces production of several other cytokines. Although TNF-α is used in clinical trials as an antitumor agent, Sigma′s cytokine, growth factor and hormone products are for research only. TNF-α and the related molecule TNF-β (LT-α) share close structural homology with 28% amino acid sequence identity and both activate the same TNF receptors, TNFR1 and TNFR2. Mouse and human TNF-α share 79% amino acid sequence identity. Unlike human TNF-α, the mouse form is glycosylated.

Unit Definition

One unit is the amount required to induce half-maximal cytolysis of L929 cells.

Physical form

Lyophilized from 10 mM sodium phosphate, pH 7.5

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H315 - H319
• Precautionary Statements: P264 - P280 - P302 + P352 - P305 + P351 + P338 - P332 + P313 - P337 + P313
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves