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T8154

Sigma-Aldrich

Trypan Blue solution

0.4%, liquid, sterile-filtered, suitable for cell culture

Empirical Formula (Hill Notation):
C34H24N6Na4O14S4
CAS Number:
Molecular Weight:
960.81
Beilstein:
4360496
MDL number:
PubChem Substance ID:
NACRES:
NA.75

Quality Level

sterility

sterile-filtered

form

liquid

storage condition

dry at room temperature

concentration

0.4%

application(s)

cell analysis: suitable
cell culture | mammalian: suitable
tissue processing: suitable

shipped in

ambient

SMILES string

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

InChI key

GLNADSQYFUSGOU-UHFFFAOYSA-J

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General description

Trypan blue is a dye used to distinguish between live and dead cells. It is a vital stain that is not absorbed by healthy viable cells, but stains cells with a damaged cell membrane. This way, only dead cells can be counted. The method is sometimes referred to as the dye exclusion method.

Application

Use to detect dead and dying cells in cytotoxicity assays and for routine assessment of cell viability and proliferative assays.

Preparation Note

Prepared in 0.81% sodium chloride and 0.06% potassium phosphate, dibasic.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Carc. 1B

Storage Class Code

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK Germany

WGK 3

Flash Point F

Not applicable

Flash Point C

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. Do the cells need to be washed to remove the culture media prior to staining with Product T8154, Trypan Blue solution?

    Trypan Blue has a greater affinity for serum proteins than for cellular protein. If the background is too dark, cells should be pelleted and resuspended in protein-free medium or salt solution prior to counting.

  3. Why is Trypan blue, Product T8154, used to determine cell viability?

    Trypan Blue is a vital stain recommended for use in estimating the proportion of viable cells in a population. The reactivity of this dye is based on the fact that the chromophore is negatively charged and does not react with the cell unless the membrane is damaged. Staining facilitates the visualization of cell morphology. Live (viable) cells do not take up the dye and dead (non-viable) cells do.

  4. Can Trypan blue, Product T8154, be used to discriminate between apoptotic and necrotic cells?

    Trypan blue will stain cells that have disrupted membranes.  It cannot differentiate between permeabilized membranes caused by apoptosis or necrosis.

  5. Trypan blue, Product T8154, contains a precipitate.  Is it still good to use?

    The trypan blue solution is still good to use.  It can be warmed to dissolve the precipitate or it can be filtered to remove the precipitate.

  6. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. Why are there red fibers in Product T8154, Trypan blue solution?

    A report of fibers in Product T8154, Trypan blue solution is sufficiently common almost to be a characteristic of the product. These fibers are a cosmetic fault rather than one of functionality.  The presence of fibers may be more pronounced following cold storage or shipping.  This product should be stored at room temperature. Filtration to remove the fibers will not affect the use of the product.

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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