Merck
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T8328

Sigma-Aldrich

Anti-β-Tubulin antibody, Mouse monoclonal

~2.0 mg/mL, clone AA2, purified from hybridoma cell culture

Synonym(s):
Anti-Tubulin, beta, Beta Tubulin Antibody Sigma - Monoclonal Anti-β-Tubulin antibody produced in mouse, Beta Tubulin Antibody Sigma, Anti-TUBB
MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

AA2, monoclonal

form

buffered aqueous solution

mol wt

antigen ~50 kDa

species reactivity

rat, human, mouse, bovine

packaging

antibody small pack of 25 μL

concentration

~2.0 mg/mL

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 1-2 μg/mL using Neuro-2a cell extract

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... TUBB(203068)

Related Categories

General description

Tubulin βIII (TUBB3) is a constituent of microtubules. It is expressed in mature neurons. It is located on human chromosome 16q24.
Tubulin, a 100kDa protein is present in all cells as a heterodimer of two similar polypeptides α and β, which assemble to form microtubules. Monoclonal anti-β-tubulin antibody can be used to visualize HRP signal using enhanced chemiluminescence.
β-tubulin belongs to the subfamily of tubulins, which are the major building blocks of microtubules. β-tubulin has a molecular weight of 55kDa. β-tubulin structure is characterized with core of two β-sheets enclosed by α-helices. It also contains an N-terminal domain with the guanine nucleotide-binding region, an intermediate domain with taxol-binding site and a C-terminal domain that contains the binding surface for molecular motor proteins. Human β-tubulin consists of seven isoforms (βI (class I), βII (class II), βIII (class III), βIVa (class IVa), βIVb (class IVb), βV (class V) and βVI (class VI)).

Specificity

Mouse anti-β-tubulin antibody reacts specifically with β tubulin, types I, II, III, and IV of bovine, rat, mouse and human.

Immunogen

Purified bovine tubulin

Application

Anti-Tubulin βIII antibody has been used in western blot analysis.
Monoclonal anti-β-tubulin antibody (diluted 1: 5000) can be used as a primary antibody in immunofluorescence staining of mammalian oocytes. It can also be used in numerous immunochemical techniques like-, immunocytochemistry, immunoblotting (50kD approx), immunohistochemistry and immunoprecipitation.
Monoclonal Anti-β-Tubulin antibody produced in mouse has been used in western blotting and immunofluorescence staining.

Biochem/physiol Actions

Proper organization of microtubules mediated by tubulin is essential for several cellular functions like mitosis, meiosis, some forms of organellar movement, intracellular transport, flagellar movement and cytoskeletal functions.
Upregulation of tubulin βIII (TUBB3) shows vigorous tumor characteristics and genetic instability in urinary bladder cancer. It participates in the separation of chromosome. Individuals with congenital fibrosis of the extraocular muscle type 3 (CFEOM3) show mutations in TUBB3. βIII-tubulin modulates glucose metabolism, thereby transforms glucose starvation stress signaling to develop the multiplication of cell and survival in non-small cell lung cancer cells.
β-1 tubulin, also known as class VI or TUBB1, plays a vital role in platelet production and is considered to be a potential biomarker candidate for immune thrombocytopenia (ITP).
B2702 peptide binds to β-tubulin and inhibits natural killer (NK) cell cytotoxicity and it influences microtubule polymerization, which damages cytoskeleton organization and chaperone-like activity of tubulin. Mutation in the gene leads to various neuronal migration disorders such as lissencephaly, pachygyria and polymicrogyria malformations.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month.
For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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