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U6504

Sigma-Aldrich

Urea

suitable for electrophoresis

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Synonym(s):
Carbamide, Carbonyldiamide
Linear Formula:
NH2CONH2
CAS Number:
Molecular Weight:
60.06
Beilstein:
635724
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NB.22

Assay

≥98%

Quality Level

form

powder or crystals

technique(s)

electrophoresis: suitable

impurities

≤0.005% Ammonia

mp

132-135 °C (lit.)

solubility

H2O: 100 mg/mL

density

1.335 g/mL at 25 °C (lit.)

cation traces

Fe: ≤5 ppm
Pb: ≤5 ppm

absorption

≤0.06 at 260 at 5 M
≤0.06 at 280 at 5 M

SMILES string

NC(N)=O

InChI

1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)

InChI key

XSQUKJJJFZCRTK-UHFFFAOYSA-N

Gene Information

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This Item
U537815604U0631
Urea suitable for electrophoresis

U6504

Urea

-
Urea powder, BioReagent, for molecular biology, suitable for cell culture

U5378

Urea

Essential+ Grade
Urea puriss., meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%, 99.0-101.0% (calc. on dry substance)

15604

Urea

-
Urea BioXtra, pH 7.5-9.5 (20 °C, 5 M in H2O)

U0631

Urea

Premium Grade
assay

≥98%

assay

≥98%

assay

99.0-100.5%, 99.0-101.0% (calc. on dry substance)

assay

98.5-101.5%

solubility

H2O: 100 mg/mL

solubility

H2O: soluble 480 mg/mL, clear, colorless (8M)

solubility

H2O: soluble 480 g/L at 20 °C

solubility

H2O: 5 M at 20 °C, clear, colorless

form

powder or crystals

form

powder

form

solid

form

powder, crystals or pellets

technique(s)

electrophoresis: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

impurities

≤0.005% Ammonia

impurities

-

impurities

alkalic reac. substances, complies, ≤0.001% heavy metals (as Pb), ≤0.04% insoluble in ethanol, ≤0.05% ammonium (NH4), ≤0.1% biuret(carbamylurea)

impurities

≤0.005% Phosphorus (P), ≤0.1% Insoluble matter

Application

A chaotropic agent used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis.
Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Addition of the trinucleotide cytosine/cytosine/adenine (CCA) to the 3' end of transfer RNAs (tRNAs) is essential for translation and is catalyzed by the enzyme TRNT1 (tRNA nucleotidyl transferase), which functions in both the cytoplasm and mitochondria. Exome sequencing revealed TRNT1
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