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WGA2

Sigma-Aldrich

GenomePlex® Complete Whole Genome Amplification (WGA) Kit

Optimized kit with enzyme for amplifying a variety of DNA including FFPE tissue

NACRES:
NA.55

Quality Level

application(s)

whole genome amplification: suitable

shipped in

wet ice

storage temp.

−20°C

General description

GenomePlex Complete Whole Genome Amplification Kit utilizes a proprietary technology based on random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable library molecules flanked by universal priming sites. WGA is achieved by PCR amplification of the library molecules using universal oligonucleotide primers.

Application

GenomePlex® Complete Whole Genome Amplification (WGA) Kit has been used in the amplification of DNA. This kit is also suitable for use with downstream applications including:
  • microarray analysis
  • SNP analysis
  • STR analysis
  • DNA archiving

Features and Benefits

  • Starting material: 10 ng gDNA or damaged DNA or 1 μl blood or cheek swab
  • Expected yield: 5 - 10 μg
  • Time required: ~3 hours (14 cycles in thermocycler)
  • WGA DNA polymerase offers increased amplification accuracy resulting in more accurate downstream applications
  • Complete representation of the entire genome with minimal allele bias
  • No amplicon in the negative control reactions
  • DNA sources: whole blood, buccal swab, blood card, plant, soil, & formalin-fixed paraffin-embedded tissue (FFPE)

Other Notes

The sequences of the universal primers provided in this kit are considered proprietary.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
GenomePlex is a registered trademark of Takara Bio USA, Inc.

Kit Components Also Available Separately

Product No.
Description
SDS

  • W4502Water, Nuclease-Free Water, for Molecular Biology

Storage Class Code

10 - Combustible liquids

WGK Germany

WGK 3

  1. What is the major difference between WGA1 and WGA2 kits?

    Functionally, WGA1 and WGA2 kits are identical. The only difference between the two kits is that WGA2 is supplied with the WGA polymerase.

  2. If starting with fragmented DNA, what is the smallest size fragment which can be successfully amplified with Product WGA2, GenomePlex® Amplification Kit, and do I still need to do the fragmentation step?

    The kit works best for fragments 400 bp and larger. When starting with fragmented DNA, we recommend: (1) skipping the fragmentation heat step, although the buffer should be added, and (2) increasing the PCR cycles from 14 to 20.

  3. Can I use Product WGA2, GenomePlex® Amplification Kit, to amplify DNA from a single cell?

    Using this kit to amplify DNA from a single cell is not recommended.  We recommend using the GenomePlex Single Cell WGA Kit (WGA4) for such application.  WGA4 was developed for use with single cells and includes an optimized cell lysis protocol which has been incorporated into the fragmentation step.

  4. Can I amplify single stranded DNA with WGA2, GenomePlex® Amplification Kit?

    When starting with single stranded starting materials, we recommend (1) skipping the fragmentation heat step, although the buffer should be added and (2) increasing the PCR cycles from 14 to 20.  Note that if the ssDNA is actually cDNA from polyadenylated RNA, the kit will likely not give good representation of the input material, as the poly(T) ends constitute a large, non-random fraction.

  5. Is the WGA2 GenomePlex® Amplification Kit polymerase compatible with TA cloning?

    WGA polymerase is compatible with TA cloning with the following alteration to the PCR step: Be sure to include a 7 to 30 minute extension at 72°C after the last cycle to ensure that all PCR products are full length and 3´ adenylated.

  6. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Articles

Oligonucleotide Array CGH Analysis of a Robust Whole Genome Amplification Method

In recent years, array-based Comparative Genomic Hybridization (aCGH) has been refined to determine chromosomal changes at progressively higher resolutions. This evolving technology is, however, somewhat hampered by the large DNA input requirement—a minimum of 150,000 copies of a human genome, or 0.5 μg, are generally needed per sample to process one CGH array.

Protocols

Protocol for DNA extraction & WGA Amplification from Soil Samples

Genomic DNA from soil samples can be easily damaged by nucleases and contaminating debris resulting in low DNA yields. As a result, the researcher’s ability to perform downstream analysis may be compromised. After isolating DNA from the soil sample, the GenomePlex® Whole Genome Amplification Protocol is followed

Saliva DNA Extraction & WGA Amplification Protocol

Whole Genome Amplification can be performed on DNA extracted in many ways. We offer many products for DNA extraction, including the GenElute™ Blood Genomic DNA Kit, GenElute Mammalian Genomic DNA Miniprep Kit and the GenElute Plant Genomic DNA M iniprep.

Whole Genome Amplification from Serum or Plasma Protocol

Whole genome amplification (WGA) of plasma and serum DNA presents a unique challenge due to the small amount of nucleic acid in such samples.

Blood Card - Extraction & Amplification WGA Protocol

Blood cards provide the convenience of archiving small volumes of blood. However, many times genomic DNA from these samples is limited, This protocol provides a simple and convenient method to extract genomic DNA from a blood card. Once the DNA has been extracted, it can then be amplified using the amplification protocol

Related Content

Array CGH Analysis of Challenging Samples

In recent years, array-based Comparative Genomic Hybridization (aCGH) has been refined to determine chromosomal changes at progressively higher resolutions. This evolving technology is, however, hampered by the large DNA input requirement—a minimum of 150,000 copies of a human genome, or 0.5 μg, are generally needed per sample to rocess one CGH array.

GenomePlex® Whole Genome Amplification Kit: A High-Throughput Approach for Rapidly Amplifying Genomic DNA from a Variety of Biological Materials

GenomePlex® Whole Genome Amplification Kit: A High-Throughput Approach for Rapidly Amplifying Genomic DNA from a Variety of Biological Materials

Qualitative multiplex PCR assay for assessing DNA quality from FFPE tissues and other sources of damaged DNA

The assessment of DNA quality is a crucial first step in acquiring meaningful data from formalin-fixed paraffin-embedded (FFPE) tissues, and other sources of damaged DNA. Using intact genomic DNA is key for successful analysis of chromosomal aberrations (e.g. SNP analysis, LOH, aCGH, etc.).

Animal Tissue DNA-Extraction & WGA Amplification Protocol

GenomePlex® Whole Genome Amplification is the method of extracting DNA from the animal sample. GenomePlex® products have been used to amplify genomic DNA from chicken, porcine, bovine, fish, and shrimp source.

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