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Discovery® DSC-NH2 SPE Tube

bed wt. 50 mg, volume 1 mL, pk of 108



PE frit (20 μm)

Quality Level




bed wt., 50 mg


pk of 108


1 mL


silica gel base material (irregularly shaped, acid washed)

matrix active group

amino phase

particle size

50 μm

pore size

70 Å pore size

surface area

480 m2/g




~0.43 meq/g capacity

Featured Industry

Food and Beverages

separation technique

ion exchange
normal phase

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General description

Retention Mechanism: Normal phase or Anion-exchange
Sample Matrix Compatibility: Organic or aqueous solutions

  • Polymerically bonded, aminopropyl phase that is very polar in nature (hydrogen bonding) allowing for both normal phase and ion exchange applications
  • A weak anion exchanger with a pKa of 9.8. At pH 7.8 or below, the functional groups are positively charged
  • Ion exchange capacity is ∼ 0.43 meq/g.
  • Allows the rapid release of very strong anions such as sulfonic acids that may be retained irreversibly by strong anion exchangers
  • Can be used in some reversed-phase applications (due to ethyl spacer); however, it is predominately used as an ion-exchange or normal phase sorbent due to its polar nature

Legal Information

Discovery is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK Germany


Flash Point F

464.0 °F

Flash Point C

> 240 °C

Certificate of Analysis

Certificate of Origin

Heather M Nonhebel et al.
Functional plant biology : FPB, 47(8), 716-726 (2020-05-23)
Current understanding of the role of plant hormones during cereal grain filling is confounded by contradictory reports on hormone production that is based on poor methodology. We report here on the accurate measurement of indole-3-acetic acid (IAA) and abscisic acid...

Related Content

Solid Phase Extraction: Ion Exchange Methodology

SPE retention mechanism in this case is based on the electrostatic attraction of charged functional groups of the analyte(s) to oppositely charged functional groups on the sorbent.

Solid Phase Extraction: Normal Phase Methodology

Retention occurs through polar interaction between the sorbent and analytes. Typical sample matrices that can be employed in normal-phase SPE include hydrocarbon or fatty oils diluted in a solvent like hexane, isooctane, chlorinated solvent, THF, diethyl ether, or ethyl acetate.

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