Trizma® base is a basimetric standard used in biochemistry and molecular biology. It may be used as a buffer or as a component of mixed buffer formulations. It is essentially stable, relatively non-hygroscopic and has a high equivalent weight.
Trizma® base can be used as a buffer:
- In the evanescent absorption analysis of NADPH.
- In the autoxidation studies of oxymyoglobin.
500 g in poly bottle
1 kg in poly bottle
5 kg in poly drum
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Trizma is a registered trademark of Sigma-Aldrich Co. LLC
Vetec is a trademark of Sigma-Aldrich International GmbH