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Mouse Embryo Media

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Media products qualified for mouse embryo culture
FVB/N embryos 96 hours post-hCG induction of ovulation demonstrate morula, cavitating morula and blastocyst developmental stages

Mouse whole embryo culture is an important method employed in mammalian developmental biology and birth defects research. This technique enables longitudinal studies of explanted rodent embryos at the organogenesis stage. An essential technique for creating transgenic mice uses the transformation and injection of stably transfected mouse embryonic stem cells (ES) into mouse blastocysts. To optimize embryo collection, manipulation, and transfer techniques, we offer a complete selection of mouse embryo media and reagents.

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human (2)

synthetic (2)

bovine (1)

guinea pig (1)

mouse (1)

rabbit (1)

media (16)

bioactive small molecules (3)

sera (3)

solvents (3)

enzymes (2)

albumins (1)

SAFC (1)

Sigma-Aldrich (34)
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M2 medium
M7167

M2 medium

With HEPES, without penicillin and streptomycin, liquid, sterile-filtered, suitable for mouse embryo cell culture

Mineral oil
M5310

Mineral oil

light oil, suitable for mouse embryo cell culture, BioXtra

Water
W1503

Water

sterile filtered, suitable for embryo cell culture, BioXtra

Bovine Serum Albumin
A3311

Bovine Serum Albumin

powder, BioXtra

EmbryoMax<SUP>®</SUP> Human Tubal Fluid (HTF) (1X), liquid, for Mouse IVF
MR-070

EmbryoMax® Human Tubal Fluid (HTF) (1X), liquid, for Mouse IVF

The EmbryoMax Human Tubal Fluid (HTF) (1X), liquid designed for use with Mouse IVF is available in a 50 mL format and has been optimized and validated for Embryo Culture.

Protease from <I>Streptomyces griseus</I>
P8811

Protease from Streptomyces griseus

powder, BioReagent, suitable for mouse embryo cell culture, ≥3.5 units/mg solid

Hyaluronidase from bovine testes
H4272

Hyaluronidase from bovine testes

Type IV-S, powder, suitable for mouse embryo cell culture, 750-3000 units/mg solid

EmbryoMax<SUP>®</SUP> Advanced KSOM Embryo Medium
MR-101

EmbryoMax® Advanced KSOM Embryo Medium

EmbryoMax Advanced KSOM Medium can be used as a single medium to harvest and culture preimplantation mouse embryos, replacing the normal 2-step system where one medium is used for embryo harvest and another is used for culture.

M2 medium
MR-015

M2 medium

EmbryoMax M2 Medium (1X), Liquid, with Phenol Red

Polyvinylpyrrolidone
P0930

Polyvinylpyrrolidone

powder, BioXtra, suitable for mouse embryo cell culture

Sodium pyruvate
P4562

Sodium pyruvate

powder, BioXtra, suitable for mouse embryo cell culture

M16 Medium
M7292

M16 Medium

With sodium bicarbonate and lactic acid, without penicillin and streptomycin, liquid, sterile-filtered

Complement sera from guinea pig
S1639

Complement sera from guinea pig

lyophilized powder

EmbryoMax<SUP>®</SUP> KSOM Mouse Embryo Media
MR-106

EmbryoMax® KSOM Mouse Embryo Media

(1X), Liquid, with 1/2 Amino Acids

Tyrode′s Solution, Acidic
T1788

Tyrode′s Solution, Acidic

liquid, sterile-filtered, suitable for mouse embryo cell culture

Sodium <SC>DL</SC>-lactate solution
L7900

Sodium DL-lactate solution

synthetic, syrup, BioXtra, suitable for mouse embryo cell culture, 60 % (w/w)

EmbryoMax<SUP>®</SUP> KSOM Mouse Embryo Media
MR-121

EmbryoMax® KSOM Mouse Embryo Media

(1X), Liquid, with 1/2 Amino Acids & Phenol Red

HEPES
H6147

HEPES

BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)

PluriSTEM<SUP>®</SUP> Human ES/iPS Cell Medium
SCM130

PluriSTEM® Human ES/iPS Cell Medium

Defined small molecule based serum-free medium that enables feeder-free culture of human ES/iPS cells and allows for media exchanges every other day without compromising the morphology or long term functionality of human pluripotent stem cells.

<SC>D</SC>-(+)-Glucose
G6152

D-(+)-Glucose

suitable for mouse embryo cell culture, ≥99.5% (GC)

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Hepes-based media for embryo handling and manipulation

  • M2 media is often used for embryo collection, and for extended handling outside the culture incubator
  • FHM is a modification of the KSOM formulation, also used for manipulating embryos outside CO2 incubation environments

Bicarbonate-based media for the culture and development of embryos

  • Modified M16 media: traditional embryo culture media based on Krebs-Ringer bicarbonate buffer
  • KSOM media allows embryos to overcome the two-cell block and supports enhanced cell division.  Our proprietary formulation provides higher yields of blastocyst development

All media are tested in mouse embryo culture and manufactured using premium quality raw materials.

EmbryoMax® Advanced KSOM Medium

EmbryoMax® Advanced KSOM Medium is an all-in-one medium for the harvest and culture of mouse embryos, replacing the traditional two-step system where unique, separate formulations are required for embryo harvest and culture. Our advanced KSOM formulation enables both handling of embryos in atmospheric conditions and culturing in a CO2-controlled incubator - with a single medium.

Six Stages of Embryo Development

        

Illustration of two round cells without nuclei, enclosed in a blue circle, representing the zygote stage of embryonic development.

Day 0

Zygote

    Illustration of two round cells with blue nuclei, enclosed in a blue circle, representing the 2-cell stage of embryonic development.

    2-cell

      Illustration of four round cells with blue nuclei, enclosed in a blue circle, representing the 4-cell stage of embryonic development.

      4-cell

        Illustration of a group of round cells with hollow centers, enclosed in a blue circle.

        Multi-cell

          Illustration of a cluster of small, round cells enclosed in a blue circle, representing a morula.

          Morula

            Illustration of numerous small, round cells clustered at the bottom of a blue circle, representing a blastocyst hatching.

            Blastocyst

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