We describe a STED microscope optimized for colocalization experiments with up to three colors. Two fluorescence labels are separated by their fluorescence lifetime whereas a third channel is discriminated by the wavelength of fluorescence emission. Since it does not require
Maturation of active zone assembly by Drosophila Bruchpilot.
Fouquet, W.; et al.
The Journal of Cell Biology, 186(1), 129-145 (2009)
Chemistry (Weinheim an der Bergstrasse, Germany), 16(1), 158-166 (2009-12-02)
Fluorescent markers emitting in the red are extremely valuable in biological microscopy since they minimize cellular autofluorescence and increase flexibility in multicolor experiments. Novel rhodamine dyes excitable with 630 nm laser light and emitting at around 660 nm have been
STED microscopy reveals that synaptotagmin remains clustered after synaptic vesicle exocytosis.
Willig K.I.; et al.
Nature, 440(7086), 935-939 (2006)
Fast, super resolution imaging via Bessel-beam stimulated emission depletion microscopy.
Zhang, P.; Goodwin, P.M.; Werner, J.H.
Optics Express, 22(10), 12398-12409 (2014)
Experimental Proof of Concept of Nanoparticle-Assisted STED.
Sonnefraud, Y.; et al
Nano Letters, 14(8), 4449-4453 (2014)
STED microscopy resolves nanoparticle assemblies.
Willig K.I.; et al.
New Journal of Physics, 8(6), 106-106 (2006)
Block Copolymer Nanostructures Mapped by Far-Field Optics.
We report on a straightforward yet powerful implementation of stimulated emission depletion (STED) fluorescence microscopy providing subdiffraction resolution in the far-field. Utilizing the same super-continuum pulsed laser source both for excitation and STED, this implementation of STED microscopy avoids elaborate
Frequency dependent detection in a STED microscope using modulated excitation light.
Ronzitti, E.; Harke, B.; Diaspro, A.
Optics Express, 21(1), 210-210 (2013)
Size-Dependent Localization and Quantitative Evaluation of the Intracellular Migration of Silica Nanoparticles in Caco-2 Cells.
Schubbe, S.; et al.
Chemistry of Materials, 24(5), 914-923 (2012)
2PE-STED Microscopy with a Single Ti. Sapphire Laser for Reduced Illumination.
Li, Q.; Wang, Y.; et al
PLoS ONE, 9(2), e88464-e88464 (2014)
Spatial organization of proteins in metastasizing cells.
Stimulated emission depletion (STED) microscopy usually employs a scanning excitation beam that is superimposed by a donut-shaped STED beam for keeping the fluorophores at the periphery of the excitation spot dark. Here, we introduce a simple birefringent device that produces
Nanoscale organization of nicotinic aceylcholine receptors by stimulated emission depletion microscopy.
Kellner, R.R., et al.
Neuroscience, 144(1), 135-143 (2007)
Sub-diffraction imaging of nitrogen-vacancy centers in diamond by stimulated emission depletion and structured illumination
Xusan, Y., et al.
Royal Society of Chemistry Advances, 4(22), 11305-11305 (2014)
Post-fusion structural changes and their roles in exocytosis and endocytosis of dense-core vesicles.
Chiang, HC.; et al
Nature Communications, 5, 3356-3356 (2014)
Two-photon excitation STED microscopy
Moneron, G.; Hell, S. W.
Optics Express, 17(17), 14567-14573 (2009)
STED nanoscopy combined with optical tweezers reveals protein dynamics on densely covered DNA.
Heller, I.; et al.
Nature Methods, 10(9), 910-916 (2013)
A novel nanoscopic tool by combining AFM with STED microscopy.
Harke, B.; et al
Optical Nanoscopy, 1(1), 3-3 (2012)
STED imaging of green fluorescent nanodiamonds containing nitrogen-vacancy-nitrogen centers.
Laporte, G.; Psaltis, D.
Biomedical Optics Express, 7(1), 34-44 (2016)
Self-Calibrated Line-Scan STED-FCS to Quantify Lipid Dynamics in Model and Cell Membranes.
Benda, A.; Ma, Y.; Gaus, K.
Biophysical Journal, 108(3), 596-609 (2015)
Reorganization of Lipid Diffusion by Myelin Basic Protein as Revealed by STED Nanoscopy.
Steshenko, O.; et al.
Biophysical Journal, 110(11), 2441-2450 (2016)
Superresolution and Fluorescence Dynamics Evidence Reveal That Intact Liposomes Do Not Cross the Human Skin Barrier.
Dreier, J.; S?rensen, J. A.; Brewer, J. R.
PLoS ONE, 11(1) (2016)
Comparing video-rate STED nanoscopy and confocal microscopy of living neurons.
Lauterbach, M. A.; et al.
Journal of Biophotonics, 3(7), 417-424 (2010)
Naked Dense Bodies Provoke Depression.
Hallermann, S.; et al.
The Journal of Neuroscience, 30(43), 14340-14345 (2010)
We report stimulated emission depletion (STED) fluorescence microscopy with continuous wave (CW) laser beams. Lateral fluorescence confinement from the scanning focal spot delivered a resolution of 29-60 nm in the focal plane, corresponding to a 5-8-fold improvement over the diffraction
Analysis of replication factories in human cells by super-resolution light microscopy.