Showing 1-30 of 280 results for "

09735

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Shuang-Qing Zhang
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 978-979, 24-28 (2014-12-23)
Icaritin (ICT), a bioactive metabolite of prenylflavonoids from genus Epimedium, has displayed potential benefits for the treatment of osteoporosis, prostate cancer, liver cancer, renal cancer and breast cancer. To investigate the quantity of ICT in bones in vivo, a simple...
Stephen W C Chung et al.
Journal of agricultural and food chemistry, 62(25), 5805-5811 (2014-06-06)
This paper reports the development of a method for the quantitative analysis of perfluorinated compounds (PFCs), including C6-C14 perfluorinated carboxylic acids (PFCAs) and C4-C12 perfluorinated sulfonates (PFSAs), in fish and fatty foods by ultraperformance liquid chromatography-electrospray ionization-tandem mass spectrometry (UPLC-ESI-MS/MS)...
Thomas Bock et al.
Nucleic acids research, 42(22), 13525-13533 (2014-11-16)
The thermophilic fungus Chaetomium thermophilum holds great promise for structural biology. To increase the efficiency of its biochemical and structural characterization and to explore its thermophilic properties beyond those of individual proteins, we obtained transcriptomics and proteomics data, and integrated...
Xiaomei Teng et al.
Journal of separation science, 38(1), 50-59 (2014-10-29)
A novel method for the screening of 151 drugs of abuse and toxic compounds in human whole blood has been developed and validated by online solid-phase extraction with liquid chromatography coupled to time-of-flight mass spectrometry. Analytes were extracted and separated...
Erik Helgeland et al.
PloS one, 9(10), e109279-e109279 (2014-10-22)
Despite major advances in early revascularization techniques, cardiovascular diseases are still the leading cause of death worldwide, and myocardial infarctions contribute heavily to this. Over the past decades, it has become apparent that reperfusion of blood to a previously ischemic...
Diana L Shuster et al.
The Journal of pharmacology and experimental therapeutics, 350(2), 425-434 (2014-06-06)
Gestational diabetes mellitus is a major complication of human pregnancy. The oral clearance (CL) of glyburide, an oral antidiabetic drug, increases 2-fold in pregnant women during late gestation versus nonpregnant controls. In this study, we examined gestational age-dependent changes in...
Daniela Remane et al.
Analytical and bioanalytical chemistry, 406(18), 4411-4424 (2014-05-13)
In recent years, drug-facilitated crime (DFC) has become an increasing problem. A minimum list of 80 analytes to be monitored in such cases has been proposed by the Society of Forensic Toxicologists (SOFT) including the recommended minimum performance limits (RMPL)....
Jessica Momb et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(2), 549-554 (2012-12-26)
Maternal supplementation with folic acid is known to reduce the incidence of neural tube defects (NTDs) by as much as 70%. Despite the strong clinical link between folate and NTDs, the biochemical mechanisms through which folic acid acts during neural...
Laurent Imbert et al.
Journal of analytical toxicology, 38(9), 676-680 (2014-09-05)
A liquid chromatography coupled with electrospray tandem mass spectrometry method was developed for the analysis of ethylene glycol, diethylene glycol, triethylene glycol, 1,4-butanediol, 1,2-butanediol, 2,3-butanediol, 1,2-propanediol and 1,3-propanediol, in serum after a Schotten-Baumann derivatization by benzoyl chloride. Usual validation parameters...
Julie Maublanc et al.
International journal of legal medicine, 129(2), 259-268 (2014-04-30)
Despite a non-invasive sampling, hair samples are generally collected in limited amounts for an obvious esthetic reason. In order to reduce the required quantity of samples, a multianalytes method allowing simultaneous identification and quantification of 35 psychoactive drugs was developed....
A Hafeez et al.
Poultry science, 93(7), 1754-1763 (2014-05-28)
Minerals play an important role for growth and bone stability in broilers. Thermal treatment and inclusion of organic acids in feed may affect the mineral absorption and tibial quality in broilers. The study was conducted to investigate the effect of...
Gomathinayagam Ponniah et al.
Analytical chemistry, 87(5), 2718-2726 (2015-02-04)
Oligosaccharides are critical for structural integrity, stability, and biological functions of recombinant monoclonal antibodies. It is relatively easy to characterize, quantify, and determine the impact of major glycoforms. While challenging to detect and quantify, certain low abundance oligosaccharides are highly...
Minli Zhang et al.
Journal of pharmaceutical and biomedical analysis, 100, 357-364 (2014-09-10)
Quantification of amino acid based neurotransmitters in extracellular fluids, such as those in the neuron synapse, presents a challenge to the analytical chemistry because of the absence of UV- or fluorescence-detectable functional groups and the low sensitivity in mass spectrometric...
Jan Zaloga et al.
International journal of nanomedicine, 9, 4847-4866 (2014-11-05)
The promising potential of superparamagnetic iron oxide nanoparticles (SPIONs) in various nanomedical applications has been frequently reported. However, although many different synthesis methods, coatings, and functionalization techniques have been described, not many core-shell SPION drug delivery systems are available for...
Yoon Kim et al.
Xenobiotica; the fate of foreign compounds in biological systems, 44(7), 627-634 (2014-04-18)
1. The absorption, distribution, metabolism and excretion of a novel dipeptidyl peptidase IV inhibitor, gemigliptin, were examined following single oral administration of (14)C-labeled gemigliptin to rats. 2. The (14)C-labeled gemigliptin was rapidly absorbed after oral administration, and its bioavailability was 95.2% (by...
Songlin Yu et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 960, 222-229 (2014-05-14)
We described a rapid and precise method for simultaneous quantification of eleven fatty acids in human serum cholesteryl esters (CEFAs) by liquid chromatography and tandem mass spectrometry (LC-MS/MS). After extraction of serum lipids with isopropanol, CEFAs were separated on reversed...
Jan Srbek et al.
Talanta, 130, 518-526 (2014-08-28)
In this study, direct analysis in real time-mass spectrometry (DART-MS) was assessed for the analysis of various pharmaceutical formulations with intention to summarize possible applications for the routine pharmaceutical development. As DART is an ambient ionization technique, it allows direct...
Sabine Kokarnig et al.
Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS), 29, 83-90 (2014-07-27)
Selenium metabolic patterns in the human body originating from five distinct selenium dietary sources, selenate, selenite, selenomethionine (SeMet), methylselenocysteine (MeSeCys) and selenized yeast, were investigated by performing concurrent HPLC-mass spectrometric analysis of human serum and urine. Total selenium and selenium...
Yannick Lecompte et al.
Journal of pharmaceutical and biomedical analysis, 96, 127-134 (2014-04-20)
Methoxyisoflavone (5-methyl-7-methoxyisoflavone) is a synthetic isoflavone used by bodybuilders for its ergogenic properties. A recent study demonstrated that methoxyisoflavone metabolites can induce false-positive results in urinary immunoassay screening tests for cannabinoids, and only one metabolite has been identified. To improve...
Clara Coscollà et al.
Journal of chromatography. A, 1368, 132-142 (2014-10-15)
A comprehensive strategy for the analysis of current airborne pesticides has been developed using liquid chromatography coupled to high resolution mass spectrometry. The methodology includes both quantitative target analysis and post-run target screening analysis. The quantitative method was validated after...
Eva Csuhai et al.
Journal of pharmaceutical sciences, 104(3), 1087-1098 (2015-01-07)
Despite extensive study of liposomal drug formulations, reliable predictive models of release kinetics in vitro and in vivo are still lacking. Progress in the development of robust, predictive release models has been hindered by a lack of systematic, quantitative characterization...
Roberto Pontarolo et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 965, 133-141 (2014-07-13)
The objective of this work was to develop and validate a HILIC-MS/MS method for the simultaneous determination of metformin and vildagliptin in human plasma. Chromatographic separation was achieved using an Atlantis HILIC Silica 150-mm × 2.1-mm, 3-μm particle size column...
Élida Alechaga et al.
Analytical and bioanalytical chemistry, 406(20), 4941-4953 (2014-06-03)
A novel LC-MS/MS method has been developed for the determination of 13 aminoglycoside antibiotics in meat products. Among the chromatographic columns tested, the mixed-mode Obelisc R provided the best performance. Electrospray has been used for the coupling of the LC...
Jun Ueyama et al.
Environmental health and preventive medicine, 19(6), 405-413 (2014-10-09)
Biological monitoring of organophosphorus insecticide (OP) metabolites, specifically dialkylphosphates (DAP) in urine, plays a key role in low-level exposure assessment of OP in individuals. The aims of this study are to develop a simple and sensitive method for determining four...
Christian Sattler et al.
Molecular genetics and genomics : MGG, 288(9), 413-424 (2013-06-27)
Methane is a key intermediate in the carbon cycle and biologically produced by methanogenic archaea. Most methanogens are able to conserve energy by reducing CO2 to methane using molecular hydrogen as electron donor (hydrogenotrophic methanogenesis), but several hydrogenotrophic methanogens can...
Jiake He et al.
Molecular pharmaceutics, 11(8), 2745-2754 (2014-06-25)
A novel positron emission tomography (PET) tracer, [(11)C]-rosuvastatin (RSV), was developed to dynamically and noninvasively measure hepatobiliary transport and tissue distribution of [(11)C]-RSV in rats. Male Sprague-Dawley rats were administered either an Oatp inhibitor, rifampin (RIF, 40 mg/kg iv bolus...
Nathaniel W Snyder et al.
Rapid communications in mass spectrometry : RCM, 28(16), 1840-1848 (2015-01-07)
Acyl-Coenzyme A (CoA) thioesters are the principal form of activated carboxylates in cells and tissues. They are employed as acyl carriers that facilitate the transfer of acyl groups to lipids and proteins. Quantification of medium- and long-chain acyl-CoAs represents a...
Kaori Taguchi et al.
Journal of chromatography. A, 1362, 270-277 (2014-09-10)
Chromatography techniques usually use a single state in the mobile phase, such as liquid, gas, or supercritical fluid. Chromatographers manage one of these techniques for their purpose but are sometimes required to use multiple methods, or even worse, multiple techniques...
Georgina Balcells et al.
Journal of chromatography. A, 1389, 65-75 (2015-03-10)
In order to improve the detection capabilities of anabolic androgenic steroids (AAS) in sports, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) screening method for the simultaneous detection of AAS phase I and phase II intact urinary metabolites (glucuronides and sulfates) was...
Fabiana Rached et al.
Journal of lipid research, 55(12), 2509-2520 (2014-10-25)
To evaluate functional and compositional properties of HDL in subjects from a kindred of genetic apoA-I deficiency, two homozygotes and six heterozygotes, with a nonsense mutation at APOA1 codon -2, Q[-2]X, were recruited together with age- and sex-matched healthy controls...