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Duygu Çimen et al.
Materials science & engineering. C, Materials for biological applications, 56, 318-324 (2015-08-08)
The aim of this study is to prepare megaporous dye-affinity cryogel discs for the purification of plasmid DNA (pDNA) from bacterial lysate. Poly(hydroxyethyl methacrylate) [PHEMA] cryogel discs were produced by free radical polymerization initiated by N,N,N',N'-tetramethylene diamine (TEMED) and ammonium
Maree T Smith et al.
Journal of pharmaceutical sciences, 104(7), 2388-2396 (2015-05-23)
This study was designed to assess the analgesic efficacy and systemic exposure of oxycodone administered topically in a novel tocopheryl phosphate mixture (TPM) gel formulation, to the inflamed hindpaws in a rat model of inflammatory pain. Unilateral hindpaw inflammation was
Beate Schwer et al.
RNA (New York, N.Y.), 21(6), 1135-1146 (2015-04-18)
Protein phosphatases regulate mRNA synthesis and processing by remodeling the carboxy-terminal domain (CTD) of RNA polymerase II (Pol2) to dynamically inscribe a Pol2 CTD code. Fission yeast Fcp1 (SpFcp1) is an essential 723-amino acid CTD phosphatase that preferentially hydrolyzes Ser2-PO4
Ali Nematollahzadeh et al.
Journal of chromatography. A, 1345, 154-163 (2014-05-03)
In the present work, we report a novel method for the reinforcement of hierarchically structured molecularly imprinted polymer (MIP) for the separation of human serum albumin (HSA) and immunoglobulin G (IgG) proteins under pressure driven flow conditions. The template proteins
Xiuzhen Zhu et al.
Electrophoresis, 36(13), 1507-1513 (2014-11-05)
Electrophoresis and dielectrophoresis of cells can reveal many distinct cellular properties but are often conducted separately. Herein a simultaneous strategy was proposed, and a simple method was established by making cells migrate through a cross channel under a micro video
Anna Poltronieri et al.
La Medicina del lavoro, 101(6), 403-408 (2010-12-15)
Hairdressers are exposed to irritants and allergenic compounds that may cause contact dermatitis, rhinitis and asthma. In this paper we describe the case of a female, age 33 years, who developed contact dermatitis after 10 years of exposure to ammonium
Siegfried Wagner et al.
Anticancer research, 35(1), 145-148 (2015-01-01)
Canine prostate cancer (PC) is a highly aggressive malignancy. However, in contrast to man, neither standard screening strategies nor curative therapeutic options exist for the companion animal. A prostate-specific membrane antigen (PSMA) screening as molecular marker akin to human PC
M Perez et al.
Analytica chimica acta, 890, 117-123 (2015-09-09)
A new passive sampler was designed and characterized for the determination of free copper ion (Cu(2+)) concentration in aqueous solution. Each sampling device was composed of a set of about 30 diffusive milligel (DMG) beads. Milligel beads with incorporated cation
Aurore Masson et al.
Scientific reports, 5, 16898-16898 (2015-11-19)
Today, Light Sheet Fluorescence Microscopy (LSFM) makes it possible to image fluorescent samples through depths of several hundreds of microns. However, LSFM also suffers from scattering, absorption and optical aberrations. Spatial variations in the refractive index inside the samples cause
Angels Ruyra et al.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology, 182, 55-63 (2014-12-24)
The zebrafish (Danio rerio) is a widely used model species for biomedical research and is also starting to be a model for aquaculture research. The ZFL cell line, established from zebrafish liver, has been mostly used in toxicological and ecotoxicological
Jessica R Ingram et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(36), E4975-E4984 (2015-08-26)
Calcium-dependent protein kinases (CDPKs) comprise the major group of Ca2+-regulated kinases in plants and protists. It has long been assumed that CDPKs are activated, like other Ca2+-regulated kinases, by derepression of the kinase domain (KD). However, we found that removal
Masaya Yamaguchi et al.
Journal of molecular biology, 427(8), 1748-1764 (2014-12-10)
The anaphase-promoting complex/cyclosome (APC/C) is a massive E3 ligase that controls mitosis by catalyzing ubiquitination of key cell cycle regulatory proteins. The APC/C assembly contains two subcomplexes: the "Platform" centers around a cullin-RING-like E3 ligase catalytic core; the "Arc Lamp"
Z Xishan et al.
Cancer gene therapy, 21(9), 397-404 (2014-09-13)
The aim of this study was to investigate the role and mechanism of miR-23a in the regulation of BCR/ABL and to provide a new prognostic biomarker for chronic myeloid leukemia (CML). The expression levels of miR-23a and BCR/ABL were assessed
Thanida Srisawasdi et al.
Materials science & engineering. C, Materials for biological applications, 56, 1-8 (2015-08-08)
Pure silk fibroin (SF) hydrogel and polycarbazole/silk fibroin (SF/PCZ) hydrogels were fabricated by solvent casting technique to evaluate electromechanical responses, dielectric properties, and cantilever deflection properties as functions of electric field strength, SF concentration, glutaraldehyde concentration, and PCZ concentration in
Naoki Itoh et al.
Journal of chromatography. A, 1404, 141-145 (2015-06-11)
A rapid and mild purification method for nanoparticles using the commercially available monolithic silica disk, MonoSpin(®), was developed. The nanoparticles were purified from a dispersed solution by filtration with the aid of centrifugation at 2290×g for 2min. The purification conditions
M Vergara-Barberán et al.
Food chemistry, 169, 28-33 (2014-09-23)
The improvement of protein extraction from olive leaves using an enzyme-assisted protocol has been investigated. Using a cellulase enzyme (Celluclast® 1.5L), different parameters that affect the extraction process, such as the influence and amount of organic solvent, enzyme amount, pH
Dali Sun et al.
Colloids and surfaces. B, Biointerfaces, 130, 119-125 (2015-05-04)
The effect of hydroxyl radicals (OH) and thermal annealing on an amorphous InGaZnO4 (aIGZO) film surface was investigated for manipulation of DNA immobilization. X-ray photoemission and fluorescence measurements were conducted to reveal the status of surface OH coverage and DNA
Fuchao Xu et al.
Journal of chromatography. A, 1401, 33-41 (2015-05-23)
A multi-residue analytical method was developed for the determination of a range of flame retardants (FRs), including polybrominated diphenyl ethers (PBDEs), emerging halogenated FRs (EFRs) and organophosphate FRs (PFRs), in food matrices. An ultrasonication and vacuum assisted extraction (UVAE), followed
Vijayashankar Suganya et al.
Clinica chimica acta; international journal of clinical chemistry, 450, 210-219 (2015-08-25)
Autism is a complex developmental disability for which no specific diagnostic markers have been identified so far. The present study aimed to evaluate whether there is any abnormal protein(s) excreted in the urine of autistic children by proteome analysis which
Andrew C-H Sue et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(18), 5591-5596 (2015-04-23)
Multiple organic functionalities can now be apportioned into nanoscale domains within a metal-coordinated framework, posing the following question: how do we control the resulting combination of "heterogeneity and order"? Here, we report the creation of a metal-organic framework, MOF-2000, whose
Marie-Rose Van Calsteren et al.
Carbohydrate research, 413, 115-122 (2015-06-29)
The neutral exopolysaccharide (NPS) of Lactobacillus delbrueckii subsp. bulgaricus strain OLL1073R-1 was purified and characterized. The molecular mass was 5.0×10(6) g/mol. Sugar and absolute configuration analyses gave the following composition: d-Glc, 1; d-Gal, 1.5. The NPS was also submitted to periodate
Junmo Kang et al.
Nanoscale, 7(15), 6567-6573 (2015-03-20)
Recently, carbon materials such as carbon nanotubes and graphene have been proposed as alternatives to indium tin oxide (ITO) for fabricating transparent conducting materials. However, obtaining low sheet resistance and high transmittance of these carbon materials has been challenging due
Soonmin Lim et al.
The Journal of nutritional biochemistry, 25(10), 1058-1065 (2014-07-23)
Ginger (the rhizome of Zingiber officinale Roscoe) has been used worldwide for many centuries in cooking and for treatment of several diseases. The main pharmacological properties of ginger include anti-inflammatory, antihyperglycemic, antiarthritic, antiemetic and neuroprotective actions. Recent studies demonstrated that
Saurav Bhattacharya et al.
Biomaterials, 51, 91-107 (2015-03-17)
Thymoquinone (TQ), a major active constituent of black seeds of Nigella sativa, has potential medical applications including spectrum of therapeutic properties against different cancers. However, little is known about their effect on breast cancer cell migration, which is the cause
Yoonkap Kim et al.
Small (Weinheim an der Bergstrasse, Germany), 11(32), 3995-4001 (2015-05-07)
The vertical integration of 1D nanostructures onto the 2D substrates has the potential to offer significant performance gains to flexible electronic devices due to high integration density, large surface area, and improved light absorption and trapping. A simple, rapid, and
Xilan Jiang et al.
Talanta, 141, 239-246 (2015-05-13)
In the present study, chitosan (CHI) functionalized Fe3O4 magnetic microspheres coated with polyaniline (PANI) were synthesized for the first time. The chitosan-functionalized magnetic microspheres (Fe3O4@CHI) were synthesized by a co-precipitation method, and then aniline was polymerized on the magnetic core.
Ling Yu et al.
Journal of colloid and interface science, 453, 151-158 (2015-05-20)
Poly[glycidyl methacrylate-co-poly (ethylene glycol) methacrylate] microspheres for the first time were successfully synthesized by atom transfer radical polymerization (ATRP) method at room temperature. The co-polymerization approach was investigated to delicately control the microsphere morphology and size-distribution by reaction conditions including
Li Zhou et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 21(7), 2930-2935 (2014-12-17)
Facile growth of CuS nanowires through self-assembly and their application as building blocks for near-infrared light-responsive functional films have been demonstrated. It is found that DNA is a key factor in preparing the CuS material with defined nanostructure. An exclusive
Claudio D Borsarelli et al.
Free radical biology & medicine, 53(4), 1004-1015 (2012-07-10)
Alpha-synuclein (αS), a 140 amino acid presynaptic protein, is the major component of the fibrillar aggregates (Lewy bodies) observed in dopaminergic neurons of patients affected by Parkinson's disease. It is currently believed that noncovalent oligomeric forms of αS, arising as
Zhizhan Gu et al.
Molecular biology of the cell, 25(4), 457-469 (2013-12-18)
Directional mesenchymal cell invasion in vivo is understood to be a stimulated event and to be regulated by cytokines, chemokines, and types of extracellular matrix (ECM). Instead, by focusing on the cellular response to ECM stiffness, we found that soft
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