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Vanessa de Oliveira-Carlos et al.
PloS one, 8(9), e73384-e73384 (2013-09-17)
The adult zebrash brain has a remarkable constitutive neurogenic capacity. The regulation and maintenance of its adult neurogenic niches are poorly understood. In mammals, Notch signaling is involved in stem cell maintenance both in embryonic and adult CNS. To better
Valeria Rudman-Melnick et al.
Journal of the American Society of Nephrology : JASN, 31(12), 2793-2814 (2020-10-30)
Current management of AKI, a potentially fatal disorder that can also initiate or exacerbate CKD, is merely supportive. Therefore, deeper understanding of the molecular pathways perturbed in AKI is needed to identify targets with potential to lead to improved treatment.
Karin M Kirschner et al.
The Journal of biological chemistry, 289(35), 24452-24462 (2014-07-20)
Amine oxidase copper-containing 1 (AOC1; formerly known as amiloride-binding protein 1) is a secreted glycoprotein that catalyzes the degradation of putrescine and histamine. Polyamines and their diamine precursor putrescine are ubiquitous to all organisms and fulfill pivotal functions in cell
James V McCann et al.
Genesis (New York, N.Y. : 2000), 58(7), e23369-e23369 (2020-06-17)
Extracellular vesicles (EVs) are abundant, lipid-enclosed vectors that contain nucleic acids and proteins, they can be secreted from donor cells and freely circulate, and they can be engulfed by recipient cells thus enabling systemic communication between heterotypic cell types. However
Yongjie Hou et al.
Cell reports, 34(6), 108712-108712 (2021-02-11)
The mammillary body is a hypothalamic nucleus that has important functions in memory and spatial navigation, but its developmental principles remain not well understood. Here, we identify progenitor-specific Fezf2 expression in the developing mammillary body and develop an intersectional fate-mapping
Mariann Eilertsen et al.
The Journal of comparative neurology, 522(18), 4003-4022 (2014-07-22)
The complexity of the nonvisual photoreception systems in teleosts has just started to be appreciated, with colocalization of multiple photoreceptor types with unresolved functions. Here we describe an intricate expression pattern of melanopsins in early life stages of the marine
Nicolai Ernlund Lassen et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 32(7), 1395-1405 (2017-02-09)
It is well known that bone remodeling starts with a resorption event and ends with bone formation. However, what happens in between and how resorption and formation are coupled remains mostly unknown. Remodeling is achieved by so-called basic multicellular units
Joseph L Bedont et al.
eLife, 10 (2021-06-05)
Sleep is a conserved and essential behavior, but its mechanistic and functional underpinnings remain poorly defined. Through unbiased genetic screening in Drosophila, we discovered a novel short-sleep mutant we named argus. Positional cloning and subsequent complementation, CRISPR/Cas9 knock-out, and RNAi
David Connor Hawke et al.
Frontiers in genetics, 12, 655882-655882 (2021-06-15)
Extracellular microRNA (miRNA) sequences derived from the pre-implantation embryo have attracted interest for their possible contributions to the ongoing embryonic-uterine milieu, as well as their potential for use as accessible biomarkers indicative of embryonic health. Spent culture media microdroplets used
Maëlle Jaouannet et al.
Bio-protocol, 8(6), e2766-e2766 (2018-03-20)
The spatio-temporal expression pattern of a gene provides important indications to better understand its biological function. In situ hybridization (ISH) uses a labeled complementary single-stranded RNA or DNA probe to localize gene transcripts in a whole organism, a whole organ
Joanna Kud et al.
Bio-protocol, 9(18), e3372-e3372 (2019-09-20)
In this study, we describe a standard whole mount in situ hybridization method which is used to determine the spatial-temporal expression pattern of genes from Globodera spp. Unlike more invasive radioactive labeling approaches, this technique is based on a safe
In situ hybridization methods for mouse whole mounts and tissue sections with and without additional β-galactosidase staining.
Komatsu Y, et al.
Mouse molecular embryology : methods and protocols, 1-15 (2014)
Haoran Zhang et al.
Frontiers in physiology, 11, 612230-612230 (2021-01-29)
Craniofacial morphogenesis depends on proper migration of neural crest cells and their interactions with placodes and other cell types. Hox genes provide positional information and are important in patterning the neural crest and pharyngeal arches (PAs) for coordinated formation of
Ying Yang et al.
Molecular cell, 75(6), 1188-1202 (2019-08-11)
The maternal-to-zygotic transition (MZT) is a conserved and fundamental process during which the maternal environment is converted to an environment of embryonic-driven development through dramatic reprogramming. However, how maternally supplied transcripts are dynamically regulated during MZT remains largely unknown. Herein
Iva Filova et al.
Molecular neurobiology, 57(12), 5307-5323 (2020-09-04)
Ear development requires the transcription factors ATOH1 for hair cell differentiation and NEUROD1 for sensory neuron development. In addition, NEUROD1 negatively regulates Atoh1 gene expression. As we previously showed that deletion of the Neurod1 gene in the cochlea results in axon guidance
Ayse Sumeyra Dereli et al.
Frontiers in neuroscience, 13, 1343-1343 (2020-01-11)
Long-term hypercapnia is associated with respiratory conditions including obstructive sleep apnea, chronic obstructive pulmonary disease and obesity hypoventilation syndrome. Animal studies have demonstrated an initial (within hours) increase in ventilatory drive followed by a decrease in this response over the
Wei Liu et al.
Molecular oncology, 14(10), 2660-2677 (2020-05-16)
Osteosarcoma is an uncommon tumor occurring in bone, accompanied by elevated incidence and reduced rate of healing. Epithelial-to-mesenchymal transition (EMT) serves as a conceptual paradigm to explain the invasion and metastasis of osteosarcoma and other cancers. Hence, developing effective therapeutic
Dan Bar Yaacov
PLoS pathogens, 18(1), e1010250-e1010250 (2022-01-19)
ADARs (adenosine deaminases acting on RNA) are known for their adenosine-to-inosine RNA editing activity, and most recently, for their role in preventing aberrant dsRNA-response by activation of dsRNA sensors (i.e., RIG-I-like receptor homologs). However, it is still unclear whether suppressing
Adam Amsterdam et al.
Molecular cancer research : MCR, 7(6), 841-850 (2009-06-18)
We screened an existing collection of zebrafish insertional mutants for cancer susceptibility by histologic examination of heterozygotes at 2 years of age. As most mutants had no altered cancer predisposition, this provided the first comprehensive description of spontaneous tumor spectrum
Asmini Athman et al.
Plant methods, 10, 29-29 (2014-09-25)
An important step in characterising the function of a gene is identifying the cells in which it is expressed. Traditional methods to determine this include in situ hybridisation, gene promoter-reporter fusions or cell isolation/purification techniques followed by quantitative PCR. These
Liang Yin et al.
Bio-protocol, 10(23), e3835-e3835 (2021-03-05)
Ribosomes are an integral part of cellular life. They are complex molecular machines consisting of multiple ribosomal proteins and RNAs. To study different aspects of ribosome composition, many methods have been developed over the decades. Here, we describe how to
Yi Zhang et al.
The Journal of clinical investigation, 125(10), 3782-3794 (2015-10-02)
Primary pain and touch sensory neurons not only detect internal and external sensory stimuli, but also receive inputs from other neurons. However, the neuronal derived inputs for primary neurons have not been systematically identified. Using a monosynaptic rabies viruses-based transneuronal
Lihui Duan et al.
Neuron, 100(1), 183-200 (2018-10-03)
Acute infection, if not kept in check, can lead to systemic inflammatory responses in the brain. Here, we show that within 2 hr of systemic inflammation, PDGFRβ mural cells of blood vessels rapidly secrete chemokine CCL2, which in turn increases total neuronal
Sofiane Hamidi et al.
Development (Cambridge, England), 147(3) (2020-02-06)
The pluripotent epiblast gives rise to all tissues and organs in the adult body. Its differentiation starts at gastrulation, when the epiblast generates mesoderm and endoderm germ layers through epithelial-mesenchymal transition (EMT). Although gastrulation EMT coincides with loss of epiblast
Lauren M Saunders et al.
eLife, 8 (2019-05-30)
Thyroid hormone (TH) regulates diverse developmental events and can drive disparate cellular outcomes. In zebrafish, TH has opposite effects on neural crest derived pigment cells of the adult stripe pattern, limiting melanophore population expansion, yet increasing yellow/orange xanthophore numbers. To
Katherine Leon et al.
Nature communications, 11(1), 194-194 (2020-01-12)
Many drugs target the extracellular regions (ECRs) of cell-surface receptors. The large and alternatively-spliced ECRs of adhesion G protein-coupled receptors (aGPCRs) have key functions in diverse biological processes including neurodevelopment, embryogenesis, and tumorigenesis. However, their structures and mechanisms of action
Stacey L Peek et al.
iScience, 25(2), 103814-103814 (2022-02-25)
Proper gene regulation is critical for both neuronal development and maintenance as the brain matures. We previously demonstrated that Akirin2, an essential nuclear protein that interacts with transcription factors and chromatin remodeling complexes, is required for the embryonic formation of
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