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Eshmuno S Technical Documentation
treatment of the gel with 6 M hydrochloric acid at 120 °C under
pressure.
0.0000
0.0005
0.0010
0.0015
0.0020
0.0025
0 50 100 150 200 250 300 350
User Guide - PLN70,PLN350
Resuspend cells in 200 µL resuspension solution.
Pipet or vortex.
3. Add 200 µL lysis solution. Invert gently to mix. Allow
to clear for ≤ 5 min.
Prepare Cleared Lysate
4. Add 350 µL neutralization
S.D.S. Purified Water Storage and Distribution System
S.D.S. 200 System: 200 liters
S.D.S. 350 System: 350 liters
Pump Performance*
30 l/min (8 gal/min) at 3 bar
(43 psi) pressure
Operating Weight**
S.D.S. 200 System: 250 kg (551 lb)
S.D.S. 350 System
S.D.S. and Accessories
during storage.
1
2
3
4
5
6
7
8
8
5
Specifications
Characteristic S.D.S 200 S.D.S 350
Water Volume 200 Liter (53
User Guide - NA0150, NA0160
cells in 200 µL Resuspension Solution.
Pipet or vortex to mix.
3. Add 200 µL Lysis Solution. Gently invert 6-8 times
to mix. Allow to clear for ≤ 5 min.
Prepare Cleared Lysate
4. Add 350
Bulletin - NA0150
up and down to mix.
• Add 200 µL of Lysis Buffer and gently invert 6–8 times to mix.
Do not vortex. Allow to clear, 3–5 minutes.
2 Prepare Cleared Lysate
• Add 350 µL of Neutralization/Binding
Bulletin - NA0150S
up and down to mix.
• Add 200 µL of Lysis Buffer and gently invert 6–8 times to mix.
Do not vortex. Allow to clear, 3–5 minutes.
2 Prepare Cleared Lysate
• Add 350 µL of Neutralization/Binding
Bulletin - NA0160
up and down to mix.
• Add 200 µL of Lysis Buffer and gently invert 6–8 times to mix.
Do not vortex. Allow to clear, 3–5 minutes.
2 Prepare Cleared Lysate
• Add 350 µL of Neutralization/Binding
Protocol: LISPRO INSULIN RIA KIT
175 L 350 L
3 ---- 175 L of 15.63 U/mL 175 L 350 L
4 ---- 175 L of 31.25 U/mL 175 L 350 L
5 ---- 175 L of 62.5
Product Information Sheet - SEP080
0 0 0.1 350
Wash
Wash
10.01
17.01
100
100
0
0
0
0
0.2
1.0
350
350
Elution 22.01 0 100 0 1.0 350
Neutralization 36.01 0 0 100 1.0 350
Re-equilibration 42.01 100 0 0 1.0 350
Stop 48.00
Bulletin - PLN350
applications.
3. Neutralize Precipitate the cell debris by adding 350 µl of the
Neutralization/Binding Solution. Gently invert the tube 4–6
times. Pellet the cell debris by centrifuging at ≥12,000 x g
Cytokine/Chemokine Secretion Profiles in Differentiated Mouse Muscle Cells
100%
150%
200%
250%
300%
350%
400%
450%
Day 0 Day 1 Day 3 Day 5
Exp#1
Exp#2
User Guide-RAB1144
zero standard (0 pg/mL).
150 µL 200 µL 200 µL 200 µL 200 µL 200 µL 200 µL
Std 1 Std 2 Std 3 Std 4 Std 5 Std 6 Std 7 0 Std
Diluent
volume
Std
Data Sheet (CoQ) - 1.14739
mg/l NH4-N]
0.000 0.500 1.000 1.500 2.000
50
0
200
20
350
300
400
0
50
100
150
200
250
300
350
400
High Throughput Screening Protocol Eshmuno® CMX Resin
supernatant via vacuum (e.g. 350 mbar for 1 minute) or
centrifugation (e.g. 1200 rcf for 3 minutes); adjust parameter if
residual liquid remains in filter plate.
6. Add 200 µL of equilibration buffer
RNA-Binding Protein Immunoprecipitation
300
200
100
2 3 4
620.5
IgG anti-SNRNP70
700
600
500
400
300
200
100
0
1
Fo
ld
E
nr
ic
hm
en
t304.69
IgG anti-PABP
400
350
Product Information Sheet - CS1130
the plate for 5 minutes at 25 °C.
5. Stop the reaction with 100 µL of Stop Solution.
6. Read the absorbance at 350 nm.
7. Plot the calibration curve (∆OD350 per minute as a
function of CPA activity
Product Information Sheet - PLN70
applications.
3. Neutralize Precipitate the cell debris by adding 350 µl of the
Neutralization/Binding Solution. Gently invert the tube 4–6
times. Pellet the cell debris by centrifuging at ≥12,000 x g
Product Information Sheet
applications.
3. Neutralize Precipitate the cell debris by adding 350 µl of the
Neutralization/Binding Solution. Gently invert the tube 4–6
times. Pellet the cell debris by centrifuging at ≥12,000 x g
Poster - Binding using SPR Biacore
0
RU
150
2e-6 4e-6 6e-6 1e-58e-6 12e-5
M
200
100
40
0
KD=12.3 µM
Concentration
R
e
sp
o
n
se
0
RU
20
2e-6 4e-6 6e-6
Comparison Between Experimental Data and Computational Fluid Dynamics in Mixing Applications
40%
20%
0%
0 50 100 150 200 250 300 350 400
N
o
rm
al
iz
ed
c
o
n
d
u
ct
iv
it
y
Time (seconds)
A: Mobius® MIX 100 System, 54 L 120 rpm
Flyer: Clarification of mammalian cell cultures by depth filtration
1 2 4 6 8 10 12 14 16 18 20
1 2 4 6 8 10 12 14 16 18 20
1 2 4 6 8 10 12 14 16 18 20
100 150 200 250
Surface Plasmon Resonance-based assays
100
150
200
250
300
350
-50 0 50 100 150 200 250 300 350 400 450
RU
R
es
po
ns
e
Time sTime
R
es
p
on
se
-20
0
20
40
60
80
100
120
Data Sheet - SCC551
containing 6 mL of ice-cold DMEM/F12 PLUS (Step 3).
6. Rinse the vial with 1 mL of ice-cold DMEM/F12 PLUS and add to the tube.
7. Repeat step 6.
8. Centrifuge the 15
Bright prospects - Solvents for spectroscopy Uvasol®
20
30
40
50
60
70
80
90
100
110
Transmittance [%]
200 225 250 275 300 325 350 375 400
Wavelength [nm]
0.00
0.25
User Guide - NA2010,NA2020
thoroughly
to mix.
4. Incubate at 55° C for 10 min.
5. Add 200 µL of 95-100% ethanol to lysate and vortex
thoroughly to mix.
Prepare Column
6. Assemble Column with 2 mL collection tube.
7.
Fluvoxamine and Related Substances (USP)
250
500
750
1000
33
2
0.00
0.25
0.50
0.75
0
100
200
300
Zero Line
200 250 300 350 nm 7.25 7.50 7.75 8.00 min
0
Product Information Sheet - SEP020
psi)
Injection
Wash 0 100 0 0 0.2 350
Wash 17.01 100 0 0 1.5 350
Elution 22.01 0 100 0 1.5 350
Neutralization 36.01 0 0 100 1.5 350
Re-equilibration 42.01 100 0 0 1.5 350
Stop 50.00
Method for 6.4 × 63.0
Data Sheet - G1N70
Incubate
1 min.
1 min.
1 min.
3 min.
Add
ethanol
G1N 350 G1N 70 G1N 10
Bind DNA to column
❑
Data Sheet - G1N10
Incubate
1 min.
1 min.
1 min.
3 min.
Add
ethanol
G1N 350 G1N 70 G1N 10
Bind DNA to column
❑
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